黄芩苷对SARS-COV-2侵袭的抑制作用研究
Inhibitory effect of baicalin on SARS-COV-2 invasion
耿雪 1王雪 2周倩 3苏昕宇 2李水仙 2祝清芬2
作者信息
- 1. 山东省食品药品检验研究院,国家药品监督管理局胶类产品质量评价重点实验室,中药标准创新与质量评价工程实验室,山东 济南 250101;山东中医药大学,山东 济南 250355
- 2. 山东省食品药品检验研究院,国家药品监督管理局胶类产品质量评价重点实验室,中药标准创新与质量评价工程实验室,山东 济南 250101
- 3. 山东省中医药研究院,山东 济南 250014
- 折叠
摘要
目的 在体外研究黄芩苷对新型冠状病毒(SARS-CoV-2)入侵细胞过程的抑制作用.方法 利用构建有荧光素酶报告基因的SARS-CoV-2 S蛋白假病毒系统,通过荧光素酶试剂盒检测加入黄芩苷和假病毒后Huh-7细胞中的荧光素表达变化,进而绘制病毒抑制曲线.结果 黄芩苷能显著抑制Huh-7 细胞的病毒感染率,黄芩苷和病毒提前共孵育组与直接加入组在不同浓度下的病毒抑制曲线并无显著差别,表明黄芩苷并不能与病毒直接结合,而是通过作用于病毒S蛋白介导的细胞融合过程产生抑制作用;黄芩苷在 0.125 mg·mL-1浓度时,对病毒的抑制率在 4h组显著降低,在 0h和 2h组并无显著差别,表明黄芩苷可能在病毒入侵细胞(非吸附)阶段产生抑制作用,且介导的入侵抑制阶段发生在 4h内.结论 黄芩苷可能通过介导抑制SARS-CoV-2 病毒S蛋白与细胞表面受体的融合过程,在非吸附阶段抑制病毒的入侵,发挥抗新冠病毒活性.
Abstract
Objective To study the antiviral efficacy of baicalin on SARS-COV-2 entry process in vitro.Methods The SARS-CoV-2 S pseudo-typed virions containing a luciferase reporter gene and Luciferase kit were used to detect the changes of fluorescein expression caused by baicalin in Huh-7 cells after incubation with the pseudo-typed virion,and then the virus inhibition curve is plotted.Results Baicalin can significantly inhibited the infection rate of pseudo virus.There was no signifi-cant difference in the virus inhibition curve between the baicalin&virus pre-incubation group and co-incubation at different concentrations,indicating that baicalin could not directly bind to virus,but could play an antiviral role by affecting the cell fu-sion process mediated by the virus S protein.We further found that,the inhibition rate of baicalin to virus decreased signifi-cantly in the 4 h group,but had no significant difference in the 0 h and 2 h groups at the concentration of 0.125 mg·mL-1,in-dicating that baicalin may have an inhibitory effect on virus invasion stage rather than adsorption stage,and the mediated inhi-bition stage occurred within 4 h.Conclusion Baicalin may mediate the fusion of SARS-CoV-2 S protein with cell surface re-ceptor and exert anti-SARS-CoV-2 activity by playing a role in inhibiting virus invasion in non-adsorption stage.
关键词
黄芩苷/新型冠状病毒/荧光素酶检测/SARS-CoV-2S蛋白Key words
Baicalin/SARS-CoV-2/Luciferase assay/SARS-CoV-2 S protein引用本文复制引用
基金项目
山东省自然科学基金重点项目(ZR2020KH021)
山东省食品药品检验研究院院级课题(SDIFDC-KY-2021009)
出版年
2024
国家科技期刊平台
NSTL
万方数据