胆汁制钩吻总碱抑制结肠癌细胞增殖的作用研究
Research on the suppressive effects of alkaloids of bile-processed Gelsemium elegans on colon cancer cell proliferation
王文义 1檀兴慧 2陈倪济世 2李德森 2吴水生2
作者信息
- 1. 福建中医药大学科技创新与转化中心,福建 福州 350122
- 2. 福建中医药大学药学院,福建 福州 350122
- 折叠
摘要
目的 探讨胆汁制钩吻总碱(胆钩吻总碱)体外抗肿瘤效果,旨在为钩吻减毒后存效的研究提供科学依据.方法 通过CCK-8 检测观察胆钩吻总碱对人结肠HCT-116 细胞、人神经胶质瘤U87 细胞、人肝癌HepG2 细胞、人肺癌A549 细胞的增殖作用,进一步以结肠癌HCT-116 为研究对象,以不同浓度胆钩吻总碱(50、100、200 μg·mL-1)干预HCT-116 细胞,通过流式细胞技术检测其对细胞周期阻滞的影响;Annexin V FITC/PI流式细胞术检测HCT-116 细胞凋亡情况;进一步从蛋白水平检测凋亡相关蛋白表达.结果 在钩吻总生物碱IC50浓度下,胆钩吻总碱对U87、A549、HepG2、HCT-116 肿瘤细胞的增殖抑制率均高于钩吻总生物碱组,并且各组之间的差异具有统计学意义(P<0.01).与空白组相比,不同浓度的胆钩吻总碱处理(50、100、200 μg·mL-1)可有效降低HCT-116细胞的集落形成,并将细胞周期阻滞在G2/M期.胆钩吻总碱还能引发结肠癌HCT-116 细胞的晚期凋亡,并对凋亡相关蛋白Bax、Bcl-2、caspase-3 起到调控作用.结论 胆钩吻总碱可以通过调控结肠癌HCT-116 细胞周期和凋亡相关蛋白的表达来抑制其增殖.
Abstract
Objective To investigate the in vitro anti-tumor effect of the alkaloids of bile-processed Gelsemium elegans(ABPG)and provide scientific basis for the study of the post-detoxification efficacy of Gelsemium elegans(GE).Methods The proliferation effects of ABPG on human colon cancer HCT-116 cells,human glioblastoma U87 cells,human hepatocellular carcinoma HepG2 cells,and human lung adenocarcinoma A549 cells were assessed using CCK-8 assay.Subsequently,focusing on colon cancer HCT-116 cells,different concentrations of ABPG(50,100,200 μg·mL-1)were used to intervene with the cells.The influence on cell cycle arrest was determined by flow cytometry.Cell apoptosis was detected using Annexin V FITC/PI flow cytometry assay.Furthermore,investigate apoptosis-related protein expression from a protein level.Results At the IC50 concentration of GE,the proliferation inhibitory rate of ABPG on U87,A549,HepG2,and HCT-116 tumor cells was higher than that of the GE group,and the differences between the groups were statistically significant(P<0.01).Compared with the control group,treatment with different concentrations of ABPG(50,100,200 μg·mL-1)effectively reduced colony formation of HCT-116 cells and arrested the cell cycle in the G2/M phase.ABPG also induced late-stage apoptosis in colon cancer HCT-116 cells and regulated apoptosis-related proteins Bax,Bcl-2,and caspase-3.Conclusion ABPG can suppress the proliferation of colon cancer HCT-116 cells by regulating the expression of cell cycle and apoptosis-related proteins.
关键词
胆汁制钩吻总碱/钩吻总碱/结肠癌/细胞增殖/细胞凋亡Key words
Alkaloids of bile-processed Gelsemium elegans/Alkaloids of Gelsemium elegans/Colon cancer/Cell proliferation/Cell apoptosis引用本文复制引用
基金项目
国家中医药局临床中药学高水平中医药重点学科建设项目(国中医药人教函[2023]85号)
福建中医药大学校管科研项目(X2021004)
出版年
2024
国家科技期刊平台
NSTL
万方数据