Analysis of the anti influenza A(H1N1)virus mechanism of Sanghuo Paidu Tea in vitro
Objective To observe the inhibitory effect of Sanghuo Paidu Tea on influenza A(H1N1)virus at the cellular level.Methods CCK-8 reagent was used to detect the cytotoxicity of Sanghuo Paidu Tea,and the anti-influenza A virus activity of Sanghuo Paidu Tea was evaluated by analyzing the changes in the expression levels of influenza A virus RNA and protein in different treatment groups.Results The maximal atoxic concentration(TC0)of Sanghuo Paidu Tea and Oseltamivir phosphate for MDCK was 4.32 mg·mL-1 and 1.02 mg·mL-1,respectively,and the 50%effective concentration(EC50)was 0.38 mg·mL-1 and 3.5 μg·mL-1.In Q-PCR and Western blotting experiments,compared to the viral group,the expression levels of influenza virus RNA and protein in infected cells treated with drugs were significantly reduced,indicating a strong inhibitory effect on the virus.Conclusion Sanghuo Paidu Tea can resist the infection of influenza A virus at the cellular level,and has further development significance.
Sanghuo Paidu TeaInfluenza A virusAntiviral effectOseltamivir
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