桑藿排毒茶体外抗甲型H1N1流感病毒机制分析
Analysis of the anti influenza A(H1N1)virus mechanism of Sanghuo Paidu Tea in vitro
邵秀丽 1何峰2
作者信息
- 1. 山东中医药大学药学院,山东 济南 250355
- 2. 山东中医药大学药学院,山东 济南 250355;黄冈师范学院李时珍中医药学院,湖北 黄冈 438000
- 折叠
摘要
目的 在细胞水平观察桑藿排毒茶对甲型H1N1 流感病毒的抑制作用.方法 利用CCK-8 试剂检测桑藿排毒茶的细胞毒性,通过分析不同处理组中甲型流感病毒RNA与蛋白表达水平的变化,评价该组方抗甲型流感病毒活性的高低.结果 桑藿排毒茶与磷酸奥司他韦对犬肾细胞(MDCK)的最大无毒浓度(TC0)分别为4.32、1.02 mg·mL-1,半数有效浓度(EC50)分别为 0.38 mg·mL-1、3.5 μg·mL-1.在Q-PCR与Western blotting试验中,相较于病毒组,经药物处理后的感染细胞中流感病毒RNA与蛋白的表达量均显著降低,说明其对病毒的抑制作用较强.结论 桑藿排毒茶在细胞水平可以抵抗甲型流感病毒感染,具有进一步开发意义.
Abstract
Objective To observe the inhibitory effect of Sanghuo Paidu Tea on influenza A(H1N1)virus at the cellular level.Methods CCK-8 reagent was used to detect the cytotoxicity of Sanghuo Paidu Tea,and the anti-influenza A virus activity of Sanghuo Paidu Tea was evaluated by analyzing the changes in the expression levels of influenza A virus RNA and protein in different treatment groups.Results The maximal atoxic concentration(TC0)of Sanghuo Paidu Tea and Oseltamivir phosphate for MDCK was 4.32 mg·mL-1 and 1.02 mg·mL-1,respectively,and the 50%effective concentration(EC50)was 0.38 mg·mL-1 and 3.5 μg·mL-1.In Q-PCR and Western blotting experiments,compared to the viral group,the expression levels of influenza virus RNA and protein in infected cells treated with drugs were significantly reduced,indicating a strong inhibitory effect on the virus.Conclusion Sanghuo Paidu Tea can resist the infection of influenza A virus at the cellular level,and has further development significance.
关键词
桑藿排毒茶/甲型流感病毒/抗病毒作用/奥司他韦Key words
Sanghuo Paidu Tea/Influenza A virus/Antiviral effect/Oseltamivir引用本文复制引用
基金项目
湖北省重点研发计划项目(2022BCE013)
出版年
2024
国家科技期刊平台
NSTL
万方数据