Hippocampal neurogenesis after cerebral ischemia induced by naringenin by ephrinb1-EphB2-Map2 pathway
Objective To investigate the ameliorating effect of naringenin(NAR)on endogenous hippocampal neurogenesis in mice with cerebral ischemia and its possible mechanism.Methods The Model of CI(cerebral ischemia)was established by bilateral common carotid artery occlusion(BCCAO);The pathological changes were detected by HE staining;The learning and memory functions were assessed by Morris water maze;The positive expression of BrdU,DCX and BrdU/NeuN was observed through immunofluorescence staining for detect hippocampal neurogenesis;The expressions of ephrinb1,EphB2,Map-2 were detected by RT-PCR and Western blotting,respectively.Results Compared with the Sham group,the pyramidal neurons in the hippocampal CA1 region were significantly damaged(P<0.01)and the learning and memory function was significantly decreased(P<0.01)after cerebral ischemia,suggesting that mice have cerebral ischemic injury.Meanwhile,the positive expression of BrdU,DCX and BrdU/NeuN in hippocampus of the model group was significantly increased,indicating that neurogenesis occurred in hippocampus after cerebral ischemia(P<0.01).Compared with the model group,the pathological damage in CA1 region could be significantly improved,learning and memory ability was significantly improved,and neurogenesis was further promoted after naringenin treatment.At the same time,the mRNA and protein expressions of ephrinb1,EphB2,Map-2 were up-regulated(P<0.05).Conclusion Naringenin could promote hippocampal neurogenesis and improve cognitive function after cerebral ischemia,which may be related to ephrinb1/EphB2/Map-2 signaling pathway.
万方数据
维普
