Effects of Babao Dan on autophagy and activation of NLRP3 inflammasomes in rats with cerebral ischemia-reperfusion injury
Objective To study the effect of Babao Dan(BBD)on cerebral ischemia-reperfusion injury in middle cerebral artery occlusion(MCAO)rat and its possible mechanism.Methods 45 healthy male SD rats were randomly divided into Sham group,MCAO group and BBD group.MCAO model was established after four days of continuous administration.Zea-Longa method was used to score neurological deficits 24 hours later;2,3,5-Triphenyltetrazolium chloride(TTC)staining method to detect cerebral infarct volume in rats;quantitative real-time fluorescent polymerase chain reaction(qRT-PCR)was used to detect the mRNA level of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),cyclooxygenase-2(COX-2),nitric oxide synthase(iNOS)and monocyte chemoattractant protein-1(MCP-1);The protein levels of NLR family Pyrin domain protein 3(NLRP3),interleukin-18(IL-18),microtubule-associated protein light chain 3(LC3),programmed death receptor-1(Beclin1)and mammalian target of rapamycin(mTOR)were detected by Western blotting.Results Compared with the MCAO group,BBD could significantly reduce the cerebral infarction volume and neurological function score of MCAO rats(P<0.05),and down-regulate mRNA levels of inflammatory mediators such as IL-6,IL-1β,TNF-α,iNOS,COX-2 and MCP-1(P<0.05,P<0.01).BBD can also down-regulate the protein expression of NLRP3,IL-18 and ratio of p-mTOR/mTOR,and up-regulate protein expression of Beclin1 and ratio of LC3-Ⅱ/LC3-I(P<0.05,P<0.01 and P<0.001).Conclusion BBD may reduce the release of inflammatory mediators by enhancing autophagy and inhibiting the activation of NLRP3 inflammasomes,thereby improving cerebral ischemia-reperfusion injury in MCAO rats.
万方数据
维普
