摘要
目的 研究八宝丹对大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)大鼠脑缺血再灌注损伤的作用及可能机制.方法 将健康雄性SD大鼠 45 只随机分为假手术组(Sham)、MCAO模型组以及八宝丹组,连续给药4d后建立MCAO模型,24h后采用Zea-Longa法进行神经功能评分;使用2,3,5-三苯基氯化四氮唑(TTC)染色法检测脑梗死体积;实时荧光定量聚合酶链式反应(qRT-PCR)法检测脑组织白介素-6(IL-6)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、环氧化酶-2(COX-2)、一氧化氮合酶(iNOS)、单核细胞趋化蛋白-1(MCP-1)mRNA水平;蛋白质免疫印迹法(Western blotting)检测脑组织含NOD样受体热蛋白结构域相关蛋白3(NLRP3)、白介素-18(IL-18)、微管相关蛋白轻链 3(LC3)、程序性死亡受体-1(Beclin1)、哺乳动物雷帕霉素靶蛋白(mTOR)蛋白表达水平.结果 与模型组相比,八宝丹可减小MCAO大鼠脑梗死体积并降低神经功能评分(P<0.05),下调脑内IL-6、IL-1β、TNF-α、iNOS、COX-2、MCP-1 等炎症介质的 mRNA 水平(P<0.05 或 P<0.01),降低脑组织中NLRP3、IL-18 蛋白表达水平以及p-mTOR/mTOR比值,并上调Beclin1 蛋白表达水平以及LC3-Ⅱ/LC3-Ⅰ比值(P<0.05、P<0.01、P<0.001).结论 八宝丹可能通过促进自噬和抑制NLRP3 炎症小体活化而减少炎症介质释放,进而改善MCAO大鼠脑缺血再灌注损伤.
Abstract
Objective To study the effect of Babao Dan(BBD)on cerebral ischemia-reperfusion injury in middle cerebral artery occlusion(MCAO)rat and its possible mechanism.Methods 45 healthy male SD rats were randomly divided into Sham group,MCAO group and BBD group.MCAO model was established after four days of continuous administration.Zea-Longa method was used to score neurological deficits 24 hours later;2,3,5-Triphenyltetrazolium chloride(TTC)staining method to detect cerebral infarct volume in rats;quantitative real-time fluorescent polymerase chain reaction(qRT-PCR)was used to detect the mRNA level of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),cyclooxygenase-2(COX-2),nitric oxide synthase(iNOS)and monocyte chemoattractant protein-1(MCP-1);The protein levels of NLR family Pyrin domain protein 3(NLRP3),interleukin-18(IL-18),microtubule-associated protein light chain 3(LC3),programmed death receptor-1(Beclin1)and mammalian target of rapamycin(mTOR)were detected by Western blotting.Results Compared with the MCAO group,BBD could significantly reduce the cerebral infarction volume and neurological function score of MCAO rats(P<0.05),and down-regulate mRNA levels of inflammatory mediators such as IL-6,IL-1β,TNF-α,iNOS,COX-2 and MCP-1(P<0.05,P<0.01).BBD can also down-regulate the protein expression of NLRP3,IL-18 and ratio of p-mTOR/mTOR,and up-regulate protein expression of Beclin1 and ratio of LC3-Ⅱ/LC3-I(P<0.05,P<0.01 and P<0.001).Conclusion BBD may reduce the release of inflammatory mediators by enhancing autophagy and inhibiting the activation of NLRP3 inflammasomes,thereby improving cerebral ischemia-reperfusion injury in MCAO rats.
维普
万方数据