Protection effects of patchouli alcohol on the intestine ischemia reperfusion injury in rats
Objective To investigate the protective effect of patchouli alcohol,which is the principal ingredient of Lingnan genuine medicinal herb Pogostemon cablin(Blanco)Benth.on the intestine ischemia reperfusion(IIR)injury in rats,so as to explore the new medicinal value of Pogostemon cablin(Blanco)Benth.Methods Rats were randomly divided into sham-operated group,model group,low dose,middle dose and high dose groups of Patchouli alcohol(PA).After oral administration of PA in each PA dose groups for 7 days,intestine ischemia reperfusion model was established by ligating su-perior mesenteric artery and reperfusion.The sham-operated group underwent sham-operation.After 4 hours reperfusion,the concentration of TNF-α,IL-6,LPS,SOD,MDA in small intestine were detected,the histopathological examination was scored according to the Chiu 6 grading method and the apoptosis index was calculated by TUNEL staining.Results Com-pared with the sham-operated group,the concentration of TNF-α,IL-6,LPS,SOD and MDA in model group elevated sig-nificantly(P＜0.05 or 0.01),additionally,histopathological score and apoptosis index also elevated obviously(P＜0.01).Af-ter treated with PA,the tissue lesions of small intestine were improved.The low and high dose groups of PA could conspicu-ously reduce the concentration of TNF-α,SOD and MDA.Moreover,the high dose group could suppress the IL-6 secretion and the cell apoptosis.However,in terms of LPS reduction,PA didn't have a significant effect.Conclusion It has been pre-liminarily found that PA can protect the intestine by inhibiting the cell apoptosis caused by IIR injury,reducing the oxidative stress and suppressing the production of inflammatory markers.
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