Expression and significance of programmed death receptor-1 in peripheral blood of patients with primary hepatocellular carcinoma
Objective To investigate the expression and significance of programmed death receptor-1 in primary hepa-tocellular carcinoma.Methods A retrospective analysis was performed on 70 patients with HCC treated at the 980th(Baiqiu'en International Peace)Hospital of the Joint Security Force from May 2020 to May 2022.All specimens were sec-tioned,and the expression of PD-1 in the specimens was measured by immunohistochemistry and Western blotting;The ex-pression of PD-1 in cancer tissues,paraneoplastic tissues and HCC with different degrees of differentiation was analyzed;The influence of different pathological characteristics(age,gender,TNM stage,tumor size,HBV positivity,metastasis or not,combined with ascites or not)on the expression of PD-1 was analyzed.Results PD-1 expression level in cancer tis-sues was significantly higher than that in paraneoplastic tissues,and the differences were all statistically significant(P＜0.05);PD-1 expression level in moderately differentiated HCC was significantly higher than that in highly differentiated and hypodifferentiated HCC,and the differences were all statistically significant(P＜0.05);The differences were not statisti-cally significant(P＞0.05)when comparing the highly differentiated type with the hypodifferentiated type;PD-1 positive The difference was not statistically significant(P＞0.05);PD-1 positive expression was related to TNM stage,tumor size,whether the tumor was metastatic or not,and the presence of ascites(P＜0.05);and patients with TNM stage Ⅲ-Ⅳ,tumor diameter＞6 cm,tumor metastasis and combined ascites The PD-1 positive expression rate was higher in HCC patients with TNM stage Ⅲ-Ⅳ,tumor diameter＞6 cm,metastasis and combined ascites.In addition,PD-1 expression was found to be higher in stage Ⅲ-Ⅳ hepatocellular carcinoma than in stage Ⅰ-Ⅱ hepatocellular carcinoma as determined by qPCR and Western blotting.Conclusion PD-1 was expressed at a higher level in cancer tissues and in moderately differentiated HCC;And the higher the tumour stage,the larger the diameter,the combination of metastasis and ascites,the higher the level of PD-1 expression.Therefore,PD-1 is expected to be one of the indicators to determine the stage and prognosis of HCC,providing new strategies for the diagnosis and treatment of HCC.
Hepatocellular carcinomaProgrammed death receptor-1Programmed death ligand-1Immunosuppres-sion
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