Effects and mechanism of Mahuang-Gancao couple aqueous extract on mouse asthma model induced by house dust mite
Objective To investigate the effect of Mahuang-Gancao couple aqueous extract on mouse asthma model in-duced by house dust mite(HDM),and its mechanism of action.Methods 72 SPF grade C57BL/6J female mice were ran-domly divided into 6 groups:normal control group(Normal),asthma model group(Model),low-dose Mahuang-Gancao de-coction group(MG-L),medium dose Mahuang-Gancao decoction group(MG-M),high-dose Mahuang-Gancao decoction group(MG-H),and positive control dexamethasone group(DXM),with 12 mice in each group.Establish mouse asthma model induced by House Dust Mite(HDM)and intervene with corresponding drugs.The cells in BALF were classified and counted by Wright-Giemsa Staining,H&E staining in lung tissue,the levels of IgE in serum were measured by ELISA,and the levels of IL-4,IL-5,IL-13 in lung tissue were detected by qPCR.Results MG-H and DXM all can significantly re-duce the proportion of eosinophils(P＜0.01),simultaneously increasing the number of alveolar macrophages(P＜0.05);The lung inflammation caused by HDM was relieved effectively,and restored the organizational structure;The mRNA expression of IL-4,IL-5,and IL-13 stimulated by HDM were inhibited(P＜0.05);The level of serum IgE was decreased(P＜0.005).Conclusion Mahuang-Gancao couple aqueous extract can obviously improve the asthma model induced by house dust mite in mice by high dose of decoction,its mechanism is related to the improving abnormal immune response in the lungs,inhibi-ting the secretion of Th2 cytokines and IgE,alleviating lung inflammation and airway inflammatory response.
Mahuang-Gancao coupleHouse dust mite(HDM)Mouse asthma modelInflammatory reactionLung function
万方数据
