Study on the microscopy of traditional tibetan medicine-Meconopsis quintuplinervia Regel and M.horridula Hook.f.& Thomson based on scanning electron microscope
Objective The micromorphology of alpine plant Meconopsis quintuplinervia Regel and M.horridula Hook.f.& Thomson were studied in order to provide reference for microscopic identification and resource development and utilization of this medicinal plant.Methods This study describes the plant morphology and micromorphological characteristics of leav-es,petals,anthers,filaments,pollen grains of Meconopsis quintuplinervia Regel and the petals,pollen grains of M.horridula Hook.f.& Thomson,which will provide reference for the development and utilization of the medicinal plant resources.Results The epidermal cells of Meconopsis quintuplinervia Regel leaves are irregular and covered with scaly waxy cuticle and sink-ing stomata,multicellular non-glandular trichomes were also observed.The base of non-glandular trichomes are enlarged,and there are many rows of single cells in the upper part arranged in a mosaic shape along the long axis,which is long and has branched and conical spikes.Their upper epidermis of the petals are slightly flat,the cell boundaries are obvious,and the cells in the lower epidermis are densely textured,with occasional invaginated stomata.Their pollen grains are spherical,round or nearly round in polar view,with 3 groove-shaped germination holes and granular spiny ornamentation on the outer wall.The pollen grains of Meconopsis have three types of tricolpate orientations:bipolar,circular,or equatorial,which is an important feature between two species of Meconopsis at the micro-morphological level.Conclusion The microscopic char-acteristics of leaves,stomata,non-glandular trichomes,petals and pollen grains of Meconopsis pentaphylla,and petals and pollen grains of M.horridula Hook.f.& Thomson,were observed and described from the microscopic point of view,which filled the gap in the study of micromorphology of Meconopsis and provided a reference for the sustainable utilization and de-velopment of Meconopsis pentaphylla resources.
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