首页|基于"质-量"双标法对南北五味子差异的研究

基于"质-量"双标法对南北五味子差异的研究

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目的 建立以对照药材为对照物质的定性和不依赖多种对照品定量的南、北五味子药材"质-量"双标控制方法。方法 采用高效液相色谱(HPLC)技术,以对照药材为对照物质分别建立北五味子和南五味子特征图谱,将其与供试药材进行对比来辨别药材的真伪。采用"五味子甲素"作为基准物质对南、北五味子共有特征峰化学成分相对定量进行比较。结果 北五味子中含有五味子醇甲与五味子乙素较多,南五味子中含有五味子酯甲较多。结论 该方法能清晰、快速地判断药材的真伪优劣,也可以计算出各特征峰相对含量,并对南、北五味子区分鉴别,为南北五味子的质量控制提供参考。
Based on the"quality-quantity"double standard method for the difference between the Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus
Objective To establish a"quality-quantity"double standard control method for Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus by using reference medicinal materials as control substance and don't inde-pend on a variety of reference substances.Methods The characteristic chromatograms of Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus were established by high performance liquid chromatography(HPLC)with reference me-dicinal materials as reference substances,and it was compared with the tested traditional Chinese medicinal materials to i-dentify the authenticity of the medicinal materials.Using"schisandrin A"as reference material,the relative quantitative comparison of the chemical components of the common characteristic peaks of Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus was carried out.Results Schisandrae Chinensis Fructus contains more schisandrin and schisandrin B.Schisandrae Sphenantherae Fructus contains more schisantherin A.Conclusion The method can identify Schisandra chinensis clearly and quickly without many reference substances and the relative content of each characteristic peak can also be calculated.It also distinguishes the Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus,and provides reference for the quality control of Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus.

Schisandrae Chinensis FructusSchisandrae Sphenantherae FructusReference traditional Chinese medicinal materialsSchisandrinSchisantherin ASchisandrin ASchisandrin B

王一格、张强、包永睿、王帅、李天娇、孟宪生

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辽宁中医药大学药学院,辽宁大连 116600

辽宁省中药多维分析专业创新技术中心,辽宁大连 116600

辽宁省现代中药研究工程实验室,辽宁大连 116600

北五味子 南五味子 对照药材 五味子醇甲 五味子酯甲 五味子甲素 五味子乙素

2024

药学研究
山东省药品检验所 山东省药学会

药学研究

CSTPCD
影响因子:0.653
ISSN:2095-5375
年,卷(期):2024.43(12)