Research on the"quality-quantity"dual-standard quality analysis method of Puerariae Lobatae Radix in traditional Chinese medicine based on dual-wavelength differential fusion spectrum
Objective A qualitative and quantitative"quality-quantity"dual-standard control method for Puerariae Lobatae Radix medicinal materials based on the control materials as the control substance and the quantitative control meth-od of Puerariae Lobatae Radix without relying on a variety of reference substances was established.Methods"Quality"re-fers to the use of high-performance liquid chromatography(HPLC)technology,using the control medicinal materials as the control substance to establish the characteristic map,and clarifying the authenticity of the medicinal materials by the similar-ity of characteristic peaks.The"amount"was the quantification of the reference substance"daidzein",the relative content of each characteristic peak in the test sample was calculated,and the"mean-standard deviation"was taken as the lower limit of relative content,and the advantages and disadvantages of Puerariae Lobatae Radix were clarified according to the lower limit of the relative content of characteristic peaks.Results The characteristic map and content determination method based on the dual-wavelength equal baseline difference fusion method met the requirements of the methodology.The similar-ity between the control and the tested herbs was greater than 0.950,and the lower limit of the relative content of characteris-tic peaks of the Puerariae Lobatae Radix was specified.Conclusion This method does not rely on a variety of reference substances,and can clearly and quickly judge the authenticity of medicinal materials,which provides a reference for the quality control method of Puerariae Lobatae Radix.
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