首页|低温胁迫下茶树叶片活性氧代谢及渗透调节物质含量的变化规律

低温胁迫下茶树叶片活性氧代谢及渗透调节物质含量的变化规律

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为了研究茶树对低温胁迫的生理生化反应,揭示茶树适应逆境的生理机制及其与茶树抗寒性关系,本试验以扦插苗福鼎大白和实生苗鸠坑种的两年生枝条为试材,在不同低温胁迫时间下对茶树叶片中超氧阴离子(O2-)的产生速率以及过氧化氢(H2O2)、丙二醛(MDA)、脯氨酸、可溶性蛋白和可溶性糖的含量进行了测定.结果表明,随着低温胁迫时间的延长,茶树叶片内O2-产生速率呈现先增高后降低的趋势,H2O2、MDA含量显著升高,脯氨酸含量变化无明显规律;可溶性蛋白和可溶性糖的绝对含量的增加,减缓了细胞膜透性的增加.
Variation of Active Oxygen Metabolism and Osmotic Regulators Contents in Camellia sinensis Leaves under Low-temperature Stress
In order to study the physiological and biochemical responses of tea trees to low - temperature stress, and reveal the physiological mechanism of stress adaptation and its relationship with the cold resistance of tea tree, the branches of two - year - old Camellia sinensis cv. Fudingdabai and Jiukengzhong were used as materials to determine the generation rate of superoxide anton (O-2 ) and the contents of H2O2, malondialde-hyde ( MDA), proline, soluble protein and soluble sugar in tea leaves under different treat time of cold stress. The results showed that with the prolonging of stress time, the generation rate of 0-2 increased to a certain degree and then decreased, and the contents of H2O2 and MDA increased significantly. But the variation of pro-line content was not regular. The absolute content of soluble protein and soluble sugar increased, which could slow down the increase of cell membrane permeability.

Camellia sinensisLow - temperature stressActive oxygenOsmotic regulators

时慧、王玉、周克福、丁兆堂

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青岛农业大学茶叶研究所,山东青岛266100

茶树 低温胁迫 活性氧 渗透调节物质

山东省自然科学基金山东省自然科学基金青岛市公共领域科技支撑计划山东省科技发展计划项目

ZR2010CL0212009ZRB0165410-3-3-17-nsh2011GNC11010

2012

山东农业科学
山东省农业科学院,山东农学会,山东农业大学

山东农业科学

CSTPCD
影响因子:0.578
ISSN:1001-4942
年,卷(期):2012.44(7)
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