Compared with the control parent Luhua 11(LH11),the seeds of its mutant ssm1 were smal-ler and wrinkled,and the hundred-kernel weight,productivity per plant and oil content were significantly low-er.In order to explore the causes of reduction in seed size and oil content,the expression profiles of key tran-scription factor genes in seed development such as LEC1 were studied during fertilization of zygote and different stages of seed development of ssm1 and LH11.The results showed that the expression level of AhLEC1 was lower in unfertilized ovaries(DBP0)with no significant difference between ssm1 and LH11,but was up-regu-lated in the ovaries after fertilization(DBP1)and the seeds 10 and 20 days after pegging(DAP10 and DAP20),which was significantly higher in LH11.The expression levels of AhFUS3,AhABI3,AhAGL15 and AhWRI1 were all reduced in different extent in ssm1,among which,the expression of AhWRI1 related to fatty acid synthesis and glycolysis decreased significantly.Therefore,it was speculated that the significantly down expression of AhLEC1 in ssm1 affect expression of AhFUS3,AhABI3,AhWRI1 and other genes,which lead to abnormal of embryo development and lipid accumulation in seeds.
维普
万方数据