首页|LEC1等转录因子基因在花生小种子突变体种子发育过程中的表达分析

LEC1等转录因子基因在花生小种子突变体种子发育过程中的表达分析

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花生突变体ssm1 与其对照亲本鲁花 11 号(LH11)相比，种子皱缩变小，百仁重、单株生产力和含油量显著降低。为了探究导致该突变体种子变小且含油量降低的原因，本试验研究了种子发育关键转录因子基因LCE1 等在突变体ssm1 及其对照LH11 合子受精及种子发育不同阶段的表达情况。结果表明，AhLEC1 在花生未受精子房(DBP0)时期表达量较低，且在ssm1 和对照LH11 中无明显差异，在受精后的子房(DBP1)和入土 10 d(DAP10)和 20 d(DAP20)种子中表达量上调，并且在LH11 中的表达量极显著高于突变体ssm1;转录因子基因AhFUS3、AhABI3、AhAGL15 和AhWRI1 在ssm1 中表达均有不同程度下调。其中，与脂肪酸合成和糖酵解关系密切的AhWRI1 基因在ssm1 中的表达量显著下调。因此推测，ssm1 中AhLEC1 基因的表达量显著下调，影响AhFUS3、AhABI3 和AhWRI1 等基因的表达，进而导致ssm1 种子胚胎发育和油脂积累的异常。
Expression Analysis of LEC1 and Other Transcription Factor Genes during Seed Development of Peanut Small Seed Mutant
Compared with the control parent Luhua 11(LH11),the seeds of its mutant ssm1 were smal-ler and wrinkled,and the hundred-kernel weight,productivity per plant and oil content were significantly low-er.In order to explore the causes of reduction in seed size and oil content,the expression profiles of key tran-scription factor genes in seed development such as LEC1 were studied during fertilization of zygote and different stages of seed development of ssm1 and LH11.The results showed that the expression level of AhLEC1 was lower in unfertilized ovaries(DBP0)with no significant difference between ssm1 and LH11,but was up-regu-lated in the ovaries after fertilization(DBP1)and the seeds 10 and 20 days after pegging(DAP10 and DAP20),which was significantly higher in LH11.The expression levels of AhFUS3,AhABI3,AhAGL15 and AhWRI1 were all reduced in different extent in ssm1,among which,the expression of AhWRI1 related to fatty acid synthesis and glycolysis decreased significantly.Therefore,it was speculated that the significantly down expression of AhLEC1 in ssm1 affect expression of AhFUS3,AhABI3,AhWRI1 and other genes,which lead to abnormal of embryo development and lipid accumulation in seeds.

PeanutSmall seed mutant ssm1Transcription factor gene LEC1Gene expressionSeed developmentLipid accumulation

朱秀瑾、郭凤丹、夏晗、赵传志、侯蕾

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山东师范大学生命科学学院,山东 济南 250014

山东省农业科学院农作物种质资源研究所/山东省作物遗传改良与生态生理重点实验室,山东 济南 250100

花生 小种子突变体ssm1 转录因子基因LEC1 基因表达 种子发育 油脂积累

山东省自然科学基金面上项目山东省自然科学基金面上项目山东省泰山学者特聘工程项目山东省农业科学院3237 工程项目山东省农业科学院农业科技创新工程项目山东省农业科学院农业科技创新工程项目

ZR2020MC104ZR2020MC105ts20190964CXGC2022A07CXGC2022F10

2024

10.14083/j.issn.1001-4942.2024.01.002

山东农业科学
山东省农业科学院,山东农学会,山东农业大学

山东农业科学

CSTPCD北大核心
影响因子：0.578
ISSN：1001-4942
年,卷(期)：2024.56(1)
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