山东农业科学2024,Vol.56Issue(1) :105-112.DOI:10.14083/j.issn.1001-4942.2024.01.014

喀斯特地区不同海拔对油茶根际土壤丛枝菌根真菌物种群落的影响

Effects of Different Altitudes on Diversity of Arbuscular Mycorrhizal Fungi in Rhizosphere Soil of Camellia oleifera in Karst Region

曾维军 刘燕 秦维 罗文敏
山东农业科学2024,Vol.56Issue(1) :105-112.DOI:10.14083/j.issn.1001-4942.2024.01.014

喀斯特地区不同海拔对油茶根际土壤丛枝菌根真菌物种群落的影响

Effects of Different Altitudes on Diversity of Arbuscular Mycorrhizal Fungi in Rhizosphere Soil of Camellia oleifera in Karst Region

曾维军 1刘燕 1秦维 1罗文敏1
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作者信息

  • 1. 贵州省生物研究所,贵州 贵阳 550009
  • 折叠

摘要

本研究旨在了解喀斯特地区不同海拔油茶根际土壤丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)的种群多样性及物种分布特征,以提高AMF在喀斯特地区生态修复中的应用基础.以贵州喀斯特高、低海拔地区油茶根际土壤为材料,利用Illumina MiSeq测序平台进行高通量测序,分析AMF物种多样性及差异.结果表明,喀斯特地区油茶根际土壤AMF中球囊霉属(Glomus)占主要地位,整体丰度为 87.28%;低海拔油茶根际土壤AMF群落特异OTU数和Shannon、Chao1 指数显著高于高海拔,物种多样性较为丰富,且组间AMF物种聚类较远;油茶根际土壤有机质和有效磷含量与球囊霉属真菌丰度呈显著正相关,相关系数分别为0.58、0.65,表明喀斯特的低磷土壤促进油茶根际球囊霉属的生长,增加土壤碳库,促进油茶对磷的吸收利用.其作用机理待进一步研究验证.

Abstract

This study aimed to understand the population diversity and species distribution characteristics of arbuscular mycorrhizal fungi(AMF)in the rhizosphere soil of Camellia oleifera at different altitudes in the karst regions,and enhance the foundational application of AMF in the ecological restoration of the regions.The rhizosphere soil of C.oleifera from high and low altitude areas of Guizhou karst region was collected as materi-al,and the high-throughput sequencing was performed using the Illumina MiSeq sequencing platform to ana-lyze the species diversity and differences of AMF.The results showed that the genus Glomus dominated the AMF community in the karst region with 87.28%of total abundance.The unique OTU number,and Shannon and Chao1 indexes of AMF community from low altitude were significantly higher than those from high alti-tude,indicating higher richness in species diversity;additionally,the clustering distance of AMF species was farther between groups.Significantly positive correlations between organic matter ad available phosphorus con-tents and Glomus abundance were observed with correlation coefficients as 0.58 and 0.65,respectively.It indi-cated that low-phosphorus soil in karst regions promoted the growth of Glomus in the rhizosphere of Camellia oleifera,increased the soil carbon pool,and enhanced the phosphorus absorption and utilization by Camellia oleifera,but the mechanism underlying remained to be studied and verified further.

关键词

油茶/喀斯特地区/根际土壤/丛枝菌根真菌/多样性

Key words

Camellia oleifera/Karst region/Rhizosphere soil/Arbuscular mycorrhizal fungi/Diversity

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基金项目

贵州科学院博士基金项目(黔科院R字[2021]2号)

贵州省林业科研项目(黔林科合[2022]05号)

贵州省林业科研项目(黔科合支撑[2022]重点017号)

国家自然科学基金项目(31960393)

出版年

2024
山东农业科学
山东省农业科学院,山东农学会,山东农业大学

山东农业科学

CSTPCD北大核心
影响因子:0.578
ISSN:1001-4942
参考文献量19
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