首页|连作对植烟土壤微生物群落与环境因子的影响及相关性分析

连作对植烟土壤微生物群落与环境因子的影响及相关性分析

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为探究连作对植烟土壤微生物群落和土壤环境因子的影响及其相互作用，对连作和轮作植烟土壤进行16S rRNA高通量测序，并对土壤理化性质、酶活性、酚酸类物质等环境因子进行测定。结果表明，连作植烟土壤中鞘氨醇单胞菌属(Sphingomonas)、水恒杆菌属(Mizugakiibacter)、橙色出芽单胞菌属(Gemmatimonas)、人参土黄菌属(Flavisolibacter)相对丰度增加，细菌生态网络复杂度降低;速效钾、有效磷含量增加，水解性氮、硝态氮含量降低，转化酶活性降低，酚酸物质积累。Pearson相关性分析表明，土壤速效钾、有效磷、水解性氮、硝态氮和转化酶以及酚酸等环境因子与鞘氨醇单胞菌属、水恒杆菌属、橙色出芽单胞菌属、人参土黄菌属呈显著相关。可见，连作导致植烟土壤养分失衡，影响土壤微生物群落结构，此结论可为烤烟连作障碍研究提供理论依据。
Effects of Continuous Cropping of Tobacco on Soil Microbial Community and Environmental Factors and Their Correlation Analysis
In order to investigate the effects of continuous cropping on soil microbial community and en-vironmental factors in tobacco fields,the high-throughput sequencing based on 16S rRNA was performed,and the physicochemical properties,enzyme activities and environmental factors such as phenolic acids were as-sayed using the soil samples from continuous cropping and rotation systems of tobacco.Their interactions were also analyzed.The results showed that in continuous cropping soil,the relative abundance of Sphingomonas,Mizugakiibacter,Gemmatimonas and Flavisolibacter increased,while the complexity of the bacterial ecological network decreased.The contents of available potassium and phosphorus increased,the hydrolysable nitrogen and nitrate nitrogen contents decreased,the phenolic acid compounds accumulated,and the activity of trans-forming enzymes decreased.Pearson correlation analysis indicated significant correlations of soil factors such as available potassium and phosphorus,hydrolysable nitrogen,nitrate nitrogen,transforming enzymes and phe-nolic acids with the genera Sphingomonas,Mizugakiibacter,Gemmatimonas,and Flavisolibacter.In conclu-sion,continuous cropping led to nutrient imbalance and affected microbial community structure in tobacco soil.These findings could provide a theoretical basis for understanding the obstacles of continuous cropping in to-bacco cultivation.

Tobacco soilContinuous croppingMicroorganismsEnvironmental factorsCorrelation a-nalysis

朱德伦、贾孟、朱宣全、徐照丽、王戈、王娜、邓小鹏、杜宇、周鹏、白羽祥

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云南农业大学烟草学院,云南 昆明 650500

云南省烟草农业科学研究院烟草农学研究中心,云南 昆明 650021

植烟土壤 连作 微生物 环境因子 相关性分析

国家自然科学基金项目国家自然科学基金项目国家自然科学基金项目中国烟草总公司云南省公司科技计划重点项目中国烟草总公司云南省公司科技计划重点项目

31860357321605173216030220195300002410112019530000241012

2024

10.14083/j.issn.1001-4942.2024.01.015

山东农业科学
山东省农业科学院,山东农学会,山东农业大学

山东农业科学

CSTPCD北大核心
影响因子：0.578
ISSN：1001-4942
年,卷(期)：2024.56(1)
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