In order to investigate the effects of continuous cropping on soil microbial community and en-vironmental factors in tobacco fields,the high-throughput sequencing based on 16S rRNA was performed,and the physicochemical properties,enzyme activities and environmental factors such as phenolic acids were as-sayed using the soil samples from continuous cropping and rotation systems of tobacco.Their interactions were also analyzed.The results showed that in continuous cropping soil,the relative abundance of Sphingomonas,Mizugakiibacter,Gemmatimonas and Flavisolibacter increased,while the complexity of the bacterial ecological network decreased.The contents of available potassium and phosphorus increased,the hydrolysable nitrogen and nitrate nitrogen contents decreased,the phenolic acid compounds accumulated,and the activity of trans-forming enzymes decreased.Pearson correlation analysis indicated significant correlations of soil factors such as available potassium and phosphorus,hydrolysable nitrogen,nitrate nitrogen,transforming enzymes and phe-nolic acids with the genera Sphingomonas,Mizugakiibacter,Gemmatimonas,and Flavisolibacter.In conclu-sion,continuous cropping led to nutrient imbalance and affected microbial community structure in tobacco soil.These findings could provide a theoretical basis for understanding the obstacles of continuous cropping in to-bacco cultivation.
维普
万方数据