Analysis of Differentially Expressed Genes Regulated by Resveratrol in Apple Response to Apple Ring Rot
This study aimed to screen the resistance genes of apple ring rot.After resveratrol treatment,the resistant and susceptible materials were inoculated with the pathogen of apple ring rot by acupuncture method.The samples after inoculation for 0 h and 96 h were used for differential expression gene(DEG)anal-ysis by high-throughput sequencing technology.A total of 24 libraries were constructed.A total of 173.80 Gb Clean Data were detected by transcriptome sequencing.The Clean Data of each sample reached 6.05 Gb,and the Q30 value was 90.67％and above.The DEG analysis results showed that the DEGs of resistant materials after resveratrol treatment were more than those of susceptible materials,and the DEGs at 96 h after inocula-tion were more than those at 0 h after inoculation.KEGG metabolic pathway enrichment analysis showed that the enriched pathways of disease-resistant materials were far more than those of susceptible materials,and the genes enriched in disease-resistance related pathways such as plant hormone signal transduction and plant-pathogen interaction were also more than those of susceptible materials.In addition,the DEGs of resistant ma-terials were also enriched in phenylpropanoid biosynthesis pathway and flavonoid biosynthesis pathway related to lignin synthesis.It could be seen that after resveratrol treatment,the defense mechanism in the leaves of dis-ease-resistant materials changed more abundant,so as to better cope with the infection of Botryosphaeria do-thidea.The results of this study couldprovide theoretical references for understanding the molecular mechanism of resveratrol affecting apple ring rot resistance and breeding new apple varieties with ring rot resistance.
Apple ring rotResveratrolTranscriptomeDifferential expression gene
万方数据
维普
