Effects of Different Aging Time on Population Diversity of Epiphytic Bacteria and Quality of Flue-Cured Tobacco Lamina Raw Materials
Using the flue-cured tobacco lamina raw materials aged for different time(0,12,24,36 months)from Baise of Guangxi and Chenzhou of Hunan as experimental materials,the epiphytic microbial community diversity,community composition,species differences and functions were analyzed by high-throughput sequencing technology,and the chemical quality of tobacco was detected by near-infrared spectros-copy.The results showed that the population of epiphytic bacteria in the sequenced tobacco lamina was abun-dant,and a total of 28 phyla and 548 genera were identified.The dominant bacterial phyla were Proteobacte-ria,Firmicutes and Actinobacteria,and the dominant bacterial genera were Pseudomonas,unclassified f Al-caligenaceae,Thiobacillus,Sphingomonas,Xanthomonas,Delftia and Aquamicrobium.Species differentiation analysis showed that the three genera of Sphingomonas,Dickeya,and Massilia showed significant differences among different treatments,and had the highest proportion in the tobacco samples aged 0 month.Through functional analysis,the functions of chemoheterotrophy,aerobic chemoheterotrophy,dark oxidation of sulfur compounds,dark sulfide oxidation and nitrate reduction were identified as the dominant functions,and the functional diversity composition was similar between the two regions.Chemical quality analysis showed that the organic components of tobacco lamina improved with the extension of aging time,and the epiphytic microor-ganisms accelerated the aging of tobacco leaves.In summary,the diversity of epiphytic bacterial populations in the sequenced tobacco raw materials was rich,and the bacterial population in the tobacco lamina aged 0 month had the richest diversity,community composition and functional diversity.As the aging time prolonging,mi-croorganisms reacted with organic components,which accelerated the aging of tobacco leaves.These results could provide references for further compounding microbial formulation and exploring the mechanism of tobacco aging.
Aging timeFlue-cured tobacco lamina raw materialsHigh throughput sequencingDiversi-ty analysisQuality analysis
万方数据
维普
