山东农业科学2024，Vol.56Issue(2) ：95-103.DOI:10.14083/j.issn.1001-4942.2024.02.013

基于13C同位素标记法探究连作对丹参生长及光合碳分配的影响

Effects of Continuous Cropping on Growth and Photosynthetic Carbon Distribution of Salvia miltiorrhiza Based on 13C Isotope Labeling

孟缘 付心雨 鞠吉东 周冰谦 卢恒 王晓 郭兰萍 刘伟
山东农业科学2024，Vol.56Issue(2) ：95-103.DOI:10.14083/j.issn.1001-4942.2024.02.013
✕
  • 万方数据

基于13C同位素标记法探究连作对丹参生长及光合碳分配的影响

Effects of Continuous Cropping on Growth and Photosynthetic Carbon Distribution of Salvia miltiorrhiza Based on 13C Isotope Labeling

孟缘 1付心雨 1鞠吉东 1周冰谦 2卢恒 2王晓 2郭兰萍 3刘伟2
扫码查看

作者信息

  • 1. 山东中医药大学药学院,山东 济南 250355;齐鲁工业大学(山东省科学院)/山东省分析测试中心,山东 济南 250014
  • 2. 齐鲁工业大学(山东省科学院)/山东省分析测试中心,山东 济南 250014
  • 3. 中国中医科学院中药资源中心,北京 100700
  • 折叠

摘要

以 1 年龄盆栽丹参为研究对象,设置13C脉冲标记处理与12C正常处理,应用13C脉冲标记法研究连作与非连作丹参光合碳分配规律,比较植株标记 40d后的形态学与理化指标差异,分析丹参地上部、根部以及根际土壤碳的13C丰度、碳同位素比率、13 C原子百分比以及单位干重样品的13C总量,以明确连作对丹参生长与光合作用的影响机理.结果表明,连作条件下,丹参各部分生物量与叶绿素含量明显下降,抗氧化酶活性升高;有效成分中的丹参酮Ⅰ、丹参酮ⅡA、二氢丹参酮Ⅰ以及迷迭香酸含量均降低;连作显著影响13C-光合碳分配比例,非连作丹参地上部、根部以及根际土壤中13C-光合碳比率分别为 27.14％、72.80％和 0.06％,连作丹参为 59.38％、40.59％和 0.03％.综上,连作后,丹参生长发育与次生代谢受到明显影响;光合产物向地下部的转移能力降低,导致连作丹参根部生长发育受到明显抑制;丹参光合作用的强弱是反映丹参生长状况的重要指标.

Abstract

The experiment was conducted by using 1-year-old potted Salvia miltiorrhiza as research ob-ject,and setting the 13 C pulse labeling treatment and 12 C normal treatment.The 13 C pulse labeling method was used to study the photosynthetic carbon distribution patterns of continuous and non-continuous cropping S.miltiorrhiza.The morphological and physiological differences of the plants after 30 days of labeling were recor-ded.The aboveground,root and rhizosphere soil of S.miltiorrhiza were collected,and their 13 C abundance,C isotope ratio,13 C atomic percentage and total 13 C content per unit dry weight sample were compared and ana-lyzed to clarify the effects of continuous cropping on growth and photosynthesis of S.miltiorrhiza.The results showed that under continuous cropping conditions,the biomass and chlorophyll content of various parts of S.miltiorrhiza significantly decreased,and the activity of antioxidant enzymes increased.The content of tanshi-none Ⅰ,tanshinone ⅡA,dihydrotanshinone Ⅰ and rosmarinic acid in the active ingredients decreased.The dis-tribution ratio of photosynthetic carbon in different parts of S.miltiorrhiza was in the order of root>aboveground part>rhizosphere soil.Continuous cropping significantly affected the allocation proportion of 13 C photosynthetic carbon.The proportions of 13 C photosynthetic carbon in the aboveground part,root and rhizo-sphere soil of non-continuous cropping S.miltiorrhiza were 27.14％,72.80％and 0.06％respectively,while those of continuous cropping S.miltiorrhiza were 59.38％,40.59％and 0.03％,respectively.In summary,continuous cropping significantly affected the growth and secondary metabolism of S.miltiorrhiza.The photo-synthetic carbon transferred into the roots decreased with the extension of time in continuous cropping S.milti-orrhiza,which limited the growth and development of the roots.The strength of photosynthesis was an impor-tant indicator reflecting the growth status of S.miltiorrhiza.

关键词

13C脉冲标记法/光合碳/丹参/生长代谢/连作障碍

Key words

13 C pulse labeling method/Photosynthetic carbon/Salvia miltiorrhiza/Growth metabolism/Continuous cropping obstacle

引用本文复制引用

基金项目

国家自然科学基金面上项目(82173917)

国家现代农业产业技术体系项目(CARS-21)

中央本级重大增减支项目(2060302)

山东省重点研发计划项目(2021ZDSYS12)

齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(2022PX093)

出版年

2024
山东农业科学
山东省农业科学院,山东农学会,山东农业大学

山东农业科学

CSTPCD北大核心
影响因子：0.578
ISSN：1001-4942
参考文献量28
段落导航相关论文