Method for Detecting Spatial Distribution of Antibiotics Sorption Mechanism in Soil Particulate Organic Matter at Nanoscale
Soil contamination by antibiotics and associated risks has been concerned greatly as a kind of pollution problem.Soil organic matter was major antibiotics sorbent.It's hard to quantitate the antibiotics dis-tributed in particulate organic matter(POM)by traditional methods,due to difficulties in distinguishing the carbon distribution between organic matter and antibiotics.To overcome the problems above,a method com-bined with NanoSIMS and 13 C isotopic tracer was set up to detect the spatial distribution of antibiotics onto POM at the nanoscale.Ultra-thin frozen slices of POM were made using water as embedding medium,and were transferred onto silicon wafer with an eyelash pen.Testing conditions of NanaSIMS were optimized,and image data were processed using Image J with plugin MIMI Image.The distribution of antibiotics onto POM separated from soil samples of the Yellow River Delta was detected using this established method.The results showed that 94％of total antibiotics were adsorbed on the surface of POM and 6％were diffused into POM at the nanoscale.The method established in this study could provide a new angle of view for explaining mecha-nisms of other organic pollutant adsorbed onto soil organic matter.
万方数据
维普
