首页|石榴蔗糖代谢相关酶SPS和INV基因家族鉴定与表达分析

石榴蔗糖代谢相关酶SPS和INV基因家族鉴定与表达分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
蔗糖磷酸合成酶(SPS)和蔗糖转化酶(INV)是蔗糖代谢的关键调控酶，在植物生长发育过程中起重要作用。本研究利用生物信息学和荧光定量PCR等分子手段，鉴定石榴SPS和INV基因家族成员，分析其理化性质、保守结构域、保守基序、二级结构、亚细胞定位、系统进化关系和表达模式。结果表明，从石榴基因组中鉴定出 4 个SPS基因和 11 个INV基因，其编码蛋白均为不稳定蛋白，具有典型的保守结构域，家族成员间特征motif数量和种类大致相同，蛋白结构高度保守;这些蛋白不均匀地分布在染色体上，均定位于叶绿体中，二级结构主要由α-螺旋和无规则卷曲组成。石榴SPS和INV基因家族成员间存在不同程度的亲缘关系，与巨桉同源性较高;不同SPS和INV基因在石榴果实不同发育时期的表达模式存在差异，PgINV3 在 9 月 15日(果实增大期)表达水平最高，显著高于其他时期。本研究结果对解析石榴果实中蔗糖代谢的分子机理具有重要意义。
Identification and Expression Analysis of Sucrose Phosphate Synthase(SPS)and Invertase(INV)Gene Families in Pomegranate
Sucrose phosphate synthetase(SPS)and sucrose invertase(INV)are key regulatory enzymes of sucrose metabolism and play important roles in plant growth and development.Bioinformatics and fluores-cence quantitative PCR were used to identify the SPS and INV gene family members of pomegranate and ana-lyze their physical and chemical properties,conserved domains,conserved motifs,secondary structure,sub-cellular localization,phylogenetic relationships and expression patterns in the study.The results showed that 4 SPS genes and 11 INV genes were identified from pomegranate,whose encoded proteins were all unstable ones with typical conserved domains.The number and types of motifs among the family members were roughly the same,indicating highly conserved protein structures.These members were distributed unevenly on chromo-somes and located in chloroplasts.The secondary structure of these proteins mainly consisted of α-helices and random curls.The SPS and INV gene family members of pomegranate had different degrees of relationship,and had higher homology with the gene family members of Eucalyptus grandis.The expression patterns of SPS and INV gene family members in pomegranate were different at different fruit developmental stages.The relative ex-pression level of PgINV3 was the highest on September 15(fruit enlargement stage),which was significantly higher compared with the other periods.The study results had great significance to elucidating the molecular mechanism of sucrose metabolism in pomegranate fruits.

Punica granatum L.SPS geneINV geneBiological information analysisGene expression

冯立娟、李英朋、王传增、尹燕雷、郭琳、谭伟

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枣庄学院山东省石榴精深加工工程技术研究中心/山东省石榴资源综合开发工程实验室,山东 枣庄 277160

山东省果树研究所,山东 泰安 271000

冠县店子镇农林水技术服务站,山东 冠县 252500

山东省农业科学院,山东 济南 250100

郓城县农业农村局,山东 郓城 274700

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石榴 SPS基因 INV基因 生物信息学分析 基因表达

枣庄学院山东省石榴精深加工工程技术研究中心山东省石榴资源综合开发工程实验室开放基金山东省重点研发计划山东省农科院农业科技创新工程项目山东省农科院揭榜科技难题项目

SLKF20210012022TZXD009CXGC2023A12SHJB2022-42

2024

10.14083/j.issn.1001-4942.2024.03.002

山东农业科学
山东省农业科学院,山东农学会,山东农业大学

山东农业科学

CSTPCD北大核心
影响因子：0.578
ISSN：1001-4942
年,卷(期)：2024.56(3)
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