不同浓度根皮苷对苹果砧木根系生长及生理特性的影响
Effects of Different Concentrations of Phlorizin on Root Growth and Physiological Characteristics of Apple Rootstock
李博洋 1任胜寒 1刘怀特 1王月 2张学英 1徐继忠1
作者信息
- 1. 河北农业大学园艺学院,河北 保定 071000
- 2. 保定市满城区农业农村局,河北 保定 072150
- 折叠
摘要
本试验以苹果砧木组培苗为试材,设置 0(CK)、0.1、0.5、1.0 mmol/L和 1.5 mmol/L共 5 个根皮苷浓度处理,研究砂培条件下各处理对苹果砧木组培苗生长、根系构型及根系生理生化特性的影响.结果表明,与CK相比,外施不同浓度根皮苷均抑制苹果砧木组培苗根系生长,显著降低组培苗鲜重和干重,均显著降低根长、根表面积、根体积、根平均直径和株高净增长量(0.1 mmol/L浓度处理除外),且随着根皮苷浓度升高而降幅增大.外源根皮苷胁迫导致苹果砧木组培苗根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性降低,与根系根皮苷代谢相关的查尔酮合酶、查尔酮异构酶及根皮苷水解酶活性显著降低,丙二醛(MDA)、超氧阴离子、脯氨酸含量显著升高,可溶性蛋白含量显著降低.综之,随着根皮苷浓度增加,苹果砧木组培苗根系抗氧化保护性酶活性和可溶性蛋白含量降低,MDA、超氧阴离子含量及脯氨酸含量升高,根系生长受到抑制,从而抑制苹果砧木组培苗生长.
Abstract
In this experiment,the apple rootstock tissue-cultured seedlings were used as materials to study the effects of different concentrations(0,0.1,0.5,1.0 and 1.5 mmol/L)of phlorizin on the seedling growth,root system architecture,and root physiological and biochemical characteristics under sand culture conditions.The results showed that compared with the control(CK),the root growth of apple rootstock seed-lings was significantly inhibited by applying phlorizin at different concentrations.The fresh and dry weights of tissue-cultured seedlings decreased significantly;the root length,root surface area,root volume,average root diameter and net growth amount of plant height(except 0.1 mmol/L treatment)were significantly decreased with the increase of phlorizin concentration;the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)decreased,and the activities of chalcone synthase,chalcone isomerase and phlorizin hydrolase were also significantly reduced by exogenous phlorizin stress;the contents of MDA,superoxide anion and proline increased significantly,and the soluble protein content decreased significantly.In summary,with the increase of phlorizin concentration,the activities of antioxidant protective enzymes and soluble protein con-tent decreased,the contents of MDA,superoxide anion and proline increased,so the root growth of apple root-stock tissue-cultured seedlings was inhibited and then the growth of seedlings was inhibited.
关键词
根皮苷/苹果砧木组培苗/根系构型/抗氧化酶活性Key words
Phlorizin/Tissue-cultured seedlings of apple rootstock/Root architecture/Activities of an-tioxidant enzymes引用本文复制引用
基金项目
国家苹果产业技术体系建设项目(CARS-27)
河北省重点研发计划(21326308D-02-03)
河北省现代农业产业技术体系建设项目水果创新团队项目(HBCT2018100403)
出版年
2024
国家科技期刊平台
NSTL
万方数据