Occurrence Status and Pathogen Identification of Millet Nematode Disease in Shandong Province
In recent years,millet nematode disease has occurred frequently and seriously in some areas of Shandong Province.To clarify the pathogenic species of millet nematode disease in Shandong Province,nine millet earhead samples were collected from Shandong Province in 2021-2022,and then the nematodes were isolated and obtained by tissue crushing-Behman funnel method,and identified by morphological and molecular biological meth-ods.The results showed that nematodes could be extracted from six millet earhead samples from Linyi,Jining,Li-aocheng,Jinan and Weifang cities.Through morphological identification,the six nematode populations were found to be the same species,and then the harm symptom and main morphological characteristics of female and male nematodes were described.Combined with the phylogenetic tree constructed based on rDNA-ITS and 28S D2/D3 re-gion sequences and PCR amplification with GU-F/GU-R primers specific for Aphelenchoides besseyi,the nematode pathogen was identified as A.besseyi.These results could provide references for researches on occurrence,distribu-tion and harm status of millet nematode disease in Shandong Province.
国家科技期刊平台
NSTL
万方数据
