Biological Characteristics and Complete Genome Analysis of an Ampicillin-Resistant Staphylococcus aureus Phage
This study aimed to isolate phages with high efficiency for lysing drug-resistant Staphylococcus aureus,and to provide candidate materials for developing phage biological products.In this study,using ampi-cillin-resistant S.aureus as host bacterium,the phage was isolated by double-layer plate method,and then its morphology was observed by transmission electron microscopy,followed by biological characteristics and whole genome sequencing analyses.The results showed that a S.aureus phage with the titer of 2.75×1010 PFU/mL was isolated,named as SP688.The phage had a long uncontracted tail,which belonged to Caudovirales,the long tail phage family.It was capable of lysing 7 strains of ampicillin-resistant S.aureus with the optimal com-plex number of infection as 1;it could inhibit the bacterial growth within 10 hours with stable activity at-20℃to 54℃and pH 3～11;it was sensitive to UV.The length of SP688 whole genome was 45 499 kb,defined as a circular DNA with GC content as 34.1% .A total of 64 ORFs were found,and 19 ones(29.69% )were predicted in function.This phage was a novel broad-spectrum one lysing ampicillin-resistant S.aureus,which might provide research material for phage therapy.
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