一株裂解耐氨苄西林金黄色葡萄球菌的噬菌体及其生物学特性与全基因组分析
Biological Characteristics and Complete Genome Analysis of an Ampicillin-Resistant Staphylococcus aureus Phage
张雪丽 1于浩淼 2闫振贵 2杨少华 3张亮 3杨宏军3
作者信息
- 1. 山东省农业科学院畜牧兽医研究所/农业农村部畜禽生物组学重点实验室,山东 济南 250100;山东农业大学动物科技学院,山东 泰安 271018
- 2. 山东农业大学动物科技学院,山东 泰安 271018
- 3. 山东省农业科学院畜牧兽医研究所/农业农村部畜禽生物组学重点实验室,山东 济南 250100;山东省畜禽疫病防治与繁育重点实验室,山东 济南 250100
- 折叠
摘要
本研究旨在分离能够高效裂解耐药金黄色葡萄球菌的噬菌体,为噬菌体生物制品的研制提供候选材料.以耐氨苄西林金黄色葡萄球菌为宿主菌,用双层平板法分离噬菌体,通过透射电子显微镜观察噬菌体形态,并对其进行生物学特性分析及全基因组测序分析.结果分离得到一株金黄色葡萄球菌噬菌体,效价达2.75×1010 PFU/mL,命名为SP688.该噬菌体具有长的不收缩尾巴,属长尾噬菌体科,对 7 株耐氨苄西林金葡菌均有裂解效果,最佳感染复数为 1,可在10h内抑制细菌生长,在温度-20～54℃范围和pH值3～11 之间活性稳定,对紫外线敏感.全基因组分析显示,SP688 基因组为环状DNA,全长 45 499 kb,GC含量为 34.1%;预测到 64 个开放阅读框(ORFs),其中 19 个(29.69%)被预测功能.该噬菌体是一株新型裂解耐氨苄西林金黄色葡萄球菌的广谱噬菌体,可为噬菌体治疗提供材料.
Abstract
This study aimed to isolate phages with high efficiency for lysing drug-resistant Staphylococcus aureus,and to provide candidate materials for developing phage biological products.In this study,using ampi-cillin-resistant S.aureus as host bacterium,the phage was isolated by double-layer plate method,and then its morphology was observed by transmission electron microscopy,followed by biological characteristics and whole genome sequencing analyses.The results showed that a S.aureus phage with the titer of 2.75×1010 PFU/mL was isolated,named as SP688.The phage had a long uncontracted tail,which belonged to Caudovirales,the long tail phage family.It was capable of lysing 7 strains of ampicillin-resistant S.aureus with the optimal com-plex number of infection as 1;it could inhibit the bacterial growth within 10 hours with stable activity at-20℃to 54℃and pH 3～11;it was sensitive to UV.The length of SP688 whole genome was 45 499 kb,defined as a circular DNA with GC content as 34.1% .A total of 64 ORFs were found,and 19 ones(29.69% )were predicted in function.This phage was a novel broad-spectrum one lysing ampicillin-resistant S.aureus,which might provide research material for phage therapy.
关键词
金黄色葡萄球菌/噬菌体/形态/生物学特性分析/全基因组分析Key words
Staphylococcus aureus/Phage/Morphology/Biological characteristics analysis/Whole ge-nome analysis引用本文复制引用
基金项目
现代农业产业技术体系建设项目(CARS36)
山东省重点研发计划(乡村振兴科技创新提振行动计划)(2022TZXD0021)
山东省农科院农业科技创新工程项目(CXGC2022A28)
出版年
2024
国家科技期刊平台
NSTL
万方数据