毛锤角细蜂寄生对黑腹果蝇转录组的影响
Parasitic Effects of Trichopria drosophilae on Transcriptome of Drosophila melanogaster
陈鑫雨 1何亮 2胡霞 2杨泽众 2刘晓琳 2谷希树2
作者信息
- 1. 天津师范大学生命科学学院,天津 300387;天津市农业科学院植物保护研究所,天津 300384
- 2. 天津市农业科学院植物保护研究所,天津 300384
- 折叠
摘要
黑腹果蝇是水果近成熟期的主要害虫,直接影响葡萄、蓝莓等浆果的品质和商品价值.本研究选用黑腹果蝇蛹期优势寄生蜂——毛锤角细蜂为试材,利用Illumina测序平台的RNA-Seq技术对被毛锤角细蜂寄生 6h和未寄生的黑腹果蝇蛹进行转录组测序分析.结果显示,寄生前后两组样本间共得到 294 个差异表达基因,其中上调基因 98 个,下调基因 196 个;对差异表达基因进行GO和KEGG功能富集分析,并探究寄生对寄主免疫相关基因表达的影响,发现毛锤角细蜂可能属于免疫逃避型寄生蜂.研究结果可为黑腹果蝇的生物防治提供参考.
Abstract
Drosophila melanogaster is the main pest at near ripening stage of fruits,directly affecting the quality and commercial value of berries such as grapes and blueberries.In this study,the dominant pupal par-asitic was p of D.melanogaster,Trichopria drosophilae,was selected as test material,and the RNA-Seq tech-nology based on Illumina sequencing platform was used to perform transcriptome sequencing and analysis of D.melanogaster pupae parasitized by T.drosophilae for 6 hours or not.The results showed that a total of 294 dif-ferentially expressed genes were obtained between the two groups samples of before and after parasitism,inclu-ding 98 upregulated genes and 196 downregulated genes.GO and KEGG functional enrichment analysis was conducted on the differentially expressed genes,and the impact of parasitism on host immune related genes'expression was explored.It was found that T.drosophilae might belong to the immune evasion type parasitic wasp.The results could provide references for the biological control of D.melanogaster.
关键词
毛锤角细蜂/黑腹果蝇/转录组/差异表达基因/功能富集/生物防治Key words
Trichopria drosophilae/Drosophila melanogaster/Transcriptome/Differentially expressed genes/Functional enrichment/Biological control引用本文复制引用
基金项目
天津市现代林果产业技术体系建设项目(ITTHRS2021000)
财政种业创新研究项目(2023ZYCX003)
出版年
2024
国家科技期刊平台
NSTL
万方数据