无子刺梨内生真菌拮抗活性菌株筛选及分子鉴定
Screening and Molecular Identification of Active Endophytic Fungi Strains of Rosa sterilis S.D.Shi
罗福新 1阚欢 1李永和 2赵平 3刘云 1罗旭璐2
作者信息
- 1. 西南林业大学生命科学学院,云南 昆明 650224
- 2. 云南农业大学园林园艺学院,云南 昆明 650201
- 3. 西南林业大学/西南地区林业生物资源高效利用国家林业和草原局重点实验室,云南 昆明 650224
- 折叠
摘要
为进一步开发利用无子刺梨枝条和叶片中具有生物防治潜力的内生真菌,以 2 种木腐菌和 4 种植物病原真菌为靶标,采用五点对峙法筛选对病原真菌具有拮抗活性的内生真菌菌株,并探讨活性菌株的分类地位.结果显示,菌株RSZF 36、RSZF 39 和RSYF 34 对 2 种木腐菌的抑制率均达 80%以上,菌株RSZF 34 和RSZF 36 对 4 种植物病原真菌的抑制率均大于 75%,菌株RSZF 36 对 6 种病原菌的抑制率均高于 75%,呈现强抑制能力.84 株内生真菌中有 12 株对 6 种病原菌抑制率均在 70%以上,属于活性菌株,其中 9 株来自枝条,3 株来自叶片.对 12 株活性菌株进行分子生物学鉴定,将其归属为1 门2 纲3 目3 科4 属,拟盘多毛孢属(Pestalotiopsis)为优势菌属,占活性菌株的 41.67%.综上,无子刺梨内生真菌中广泛分布着抑菌活性菌株,且不同内生菌株对不同病原菌的拮抗能力表现出一定的选择性或专一性.本研究结果可为无子刺梨内生真菌资源的开发利用提供理论参考.
Abstract
In order to further develop and utilize endophytic fungi with biological control potential in branches and leaves of Rosa sterilis,two species of wood-rotting fungi and four species of plant pathogenic fun-gi were used as target,the endophytic fungi strains with antagonistic activity were screened by five-point con-frontation method,and the classification status of the active strains was discussed.The results showed that the inhibition rate of RSZF 36,RSZF 39 and RSYF 34 against the two species of wood-rotting fungi were all over 80%,and that of RSZF 34 and RSZF 36 against the four species of plant pathogenic fungi were all over 75%.The inhibition rate of RSZF 36 against the 6 species of pathogens were all over 75%,showing strong inhibition ability.Among the 84 endophytic fungi strains,12 were active strains with the inhibition rate higher than 70%against the 6 pathogens,among which,9 from branches and 3 from leaves.The 12 active strains were identi-fied by molecular biology and classified into 1 phylum,2 classes,3 orders,3 families and 4 genera.Pestaloti-opsis was the dominant genus,accounting for 41.67%of the active strains.To sum up,antibacterial strains were widely distributed in the endophytic fungi of R.sterilis,and the antagonistic ability of different strains to different pathogens showed a certain selectivity or specificity.The results could provide theoretical references for further development and utilization of endophytic fungi resources from R.sterilis.
关键词
无子刺梨/内生真菌/拮抗活性/筛选/鉴定/植物病原菌Key words
Rosa sterilis/Endophytic fungi/Antagonistic activity/Screening/Identification/Plant path-ogenic fungi引用本文复制引用
基金项目
云南省科技计划农业联合专项(202301AK070157)
出版年
2024
国家科技期刊平台
NSTL
万方数据