首页|刺叶高山栎(Quercus spinosa)内生真菌筛选鉴定及促生作用测定

刺叶高山栎(Quercus spinosa)内生真菌筛选鉴定及促生作用测定

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  • 国家科技期刊平台
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刺叶高山栎(Quercus spinosa)是壳斗科(Fagaceae)栎属(Quercus)高山栎组常绿乔木或灌木，对极端生态环境有较强的适应能力。为了解刺叶高山栎内生真菌的促生功能作用，本研究从云南省昆明市采集刺叶高山栎的根、茎、叶等器官组织样品，采用组织分离法分离获得可培养内生真菌，继而对获得的内生真菌进行固氮、溶磷、解钾以及产吲哚乙酸(IAA)能力等促生特性测定，筛选具有多种促生特性的菌株，并对其进行分子生物学鉴定及形态学鉴定，测定其对多年生黑麦草种子萌发及盆栽幼苗生长的促生效果。结果显示，在刺叶高山栎根、茎、叶等器官组织中共分离得到 74 株内生真菌，挑选其中 11 株进行促生特性测定得出，10 株具有产IAA能力，8 株具有固氮能力，1 株(JG2)同时具备溶磷、解钾以及产IAA的能力，2 株(J06 和JJ15)具有固氮及良好的产 IAA能力。对 JG2、J06 及 JJ15 进行分子生物学及形态学鉴定得出，JG2 为 Talaromyces erythromellis，J06 为淡色生赤壳菌(Bionectria ochroleuca)，JJ15 为光炭轮菌(Daldinia eschscholtzii)。在种子萌发与盆栽试验中，JG2 菌株对多年生黑麦草幼苗均有显著的促生作用，表明JG2 菌株具有促生潜力。
Screening and Identification of Endophytic Fungi from Quercus spinosa and Determination of Their Growth-Promoting Functions
Quercus spinosa is an evergreen tree or shrub of Quercus alpine group in Fagaceae,which has strong adaptability to the extreme ecological environment.To explore the growth-promoting function of endo-phytic fungi,the samples of roots,stems and leaves of Quercus spinosa were collected from Kunming City,Yunnan Province,and culturable endophytic fungi were isolated by tissue separation method.The obtained en-dophytic fungi were tested for their growth-promoting characteristics such as nitrogen fixation,phosphorus dis-solution,potassium dissolution and indole-acetic acid(IAA)production in order to select the strains with di-verse growth-promoting characteristics,and then the molecular biological and morphological identification were conducted,and their growth-promoting effects on seed germination and potted seedling growth of Lolium pe-renne were determined.The results showed that 74 strains of endophytic fungi were isolated from the roots,stems and leaves,and 11 of them were selected for testing growth-promoting characteristics.The results showed that 10 strains had IAA-producing ability,8 strains had nitrogen-fixing ability,one strain(JG2)possessed phosphorus-dissolving,potassium-dissolving and IAA-producing abilities,and 2 strains(J06 and JJ15)had nitrogen-fixing and good IAA-producing abilities.Through the molecular biological and morphological identifi-cation,JG2 was identified as Talaromyces erythromellis,while J06 as Bionectria ochroleuca,and JJ15 as Daldi-nia eschscholtzii.In the seed germination and pot experiments,the strain JG2 had significant growth-promoting effects on Lolium perenne seedlings,which showed that it had the potential to promote growth.

Quercus spinoseEndophytic fungiMolecular biological identificationMorphological identi-ficationGrowth-promoting characteristics

杨明国、芦俊佳、田芸菁、张梓月、刘勉、勾朝金、张倩婷、古旭、杨发忠

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西南林业大学园林园艺学院,云南 昆明 650224

西南林业大学云南省森林灾害预警与控制重点实验室,云南 昆明 650233

云南生物多样性研究院,云南 昆明 650233

西南林业大学材料与化学工程学院,云南 昆明 650233

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刺叶高山栎 内生真菌 分子生物学鉴定 形态学鉴定 促生特性

国家自然科学基金云南省青年人才(培养)项目云南省农业基础研究联合专项

32060695990122074202301BD070001-091

2024

10.14083/j.issn.1001-4942.2024.04.017

山东农业科学
山东省农业科学院,山东农学会,山东农业大学

山东农业科学

CSTPCD北大核心
影响因子：0.578
ISSN：1001-4942
年,卷(期)：2024.56(4)
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