番茄颈腐根腐病病原菌鉴定及抗性品种筛选
Identification of Pathogen Causing Fusarium Crown and Root Rot and Screening Resistant Varieties in Tomato
苏晓梅 1王梦蕊 1吕宏君 1刘淑梅 1王施慧 1侯丽霞1
作者信息
- 1. 山东省农业科学院蔬菜研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,山东 济南 250100
- 折叠
摘要
番茄颈腐根腐病是设施番茄冬春生产中最严重的病害之一,严重威胁我国设施番茄的安全生产.近年来番茄颈腐根腐病在我国番茄主产区发生日益严重,培育抗病品种是防治该病最经济有效的方法.本试验采集典型症状的发病植株进行病原菌分离纯化,综合运用形态学观察、分子生物学鉴定和致病性测定方法,确定病原菌为尖孢镰刀菌番茄颈腐根腐病专化型(Fusarium oxysporum f.sp.radicis-lycopersici,Forl).之后利用分离的病原菌通过苗期人工接种鉴定结合抗性基因分子标记方法,对 32 个番茄商品种进行抗颈腐根腐病鉴定分析,结果显示有 18 个表现抗病,与分子标记Frl-ZL的鉴定结果一致,可用于番茄抗颈腐根腐病育种或生产.
Abstract
Fusarium crown and root rot(FCRR)is one of the most serious diseases threating protected tomato production in China.In recent years,the incidence of FCRR is increasing in the main producing areas of tomato in China.Cultivating resistant varieties is the most cost-effective method for preventing the disease.In this study,the pathogen was isolated from diseased tomato plants by tissue-isolation method.Based on morpho-logical characteristics,molecular identification and pathogenicity test,the causal pathogen was identified as Fusarium oxysporum f.sp.radicis-lycopersici(Forl).To explore the resistant varieties against Forl in produc-tion,the resistance of 32 varieties to Forl were identified through artificial inoculation at seedling stage and molecular marker-assisted identification of Frl gene.The results of inoculation showed 18 materials with resist-ance to FCRR,which was consistent with the identification results using molecular marker Frl-ZL.These se-lected varieties could be used in breeding or production of tomato with FCRR resistance.
关键词
番茄/颈腐根腐病/抗性鉴定/分子标记Key words
Tomato/Fusarium crown and root rot/Resistance identification/Molecular marker引用本文复制引用
基金项目
山东省自然科学基金(ZR2020QC152)
山东省重点研发计划农业良种工程项目(2022LZGC009)
中央引导地方科技发展专项(YDZX2022136)
出版年
2024
国家科技期刊平台
NSTL
万方数据