Activity and Mechanism of Sargassum fusiforme Polysaccharides against Senecavirus A Infection in PK-15 Cells
This experiment aimed to study the activity and mechanism of polysaccharides from Sargassum fusiforme against Senecavirus A(SVA)infection in PK-15 cells.Crude polysaccharides were extracted from Sargassum fusiforme by water extraction method,and then were divided into different molecular weight seg-ments by ultra filter diaphragm with different apertures.Anti-SVA activity of crude polysaccharides with differ-ent molecular weights were determined by fluorescence quantitative PCR,and then the segment with the high-est anti-SVA activity was purified by hot-water extract and precipitation with alcohol.The cytotoxicity and anti-SVA activity of the purified polysaccharide were determined again.The antiviral mechanism was investigated by administering the purified polysaccharide at different stages of the virus infection cycle.The results showed that the greater the molecular weight of the polysaccharide was,the stronger the anti-SVA activity was.Both concentrations of polysaccharide and virus showed quantity-effect relationships with antiviral activity.The ther-apeutic index of purified polysaccharide against SVA(MOI=0.1)infection in PK-15 cells was 412,and the inhibition rate of 1 mg/mL polysaccharide against SVA(MOI=10-1～104)infection in PK-15 cells ranged from 90%to 99.999%.The results of different administration times showed that the polysaccharide could per-form anti-SVA function during adsorbed or penetrating into cells and virus replication,and the strongest activi-ty occurred in adsorption or penetration inhibiting.The polysaccharide from Sargassum fusiforme after isolation and purification had strong anti-SVA activity,which could provide a reference for the development of subse-quent anti-SVA drugs.
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