Cloning of CpBEAT3 Gene Promoter from Chimonanthus praecox and Preliminary Verification of Its Interacting Protein
Benzyl alcohol acetyltransferase(BEAT)can catalyze the synthesis of benzyl acetate using benzyl alcohol and acetyl CoA as substrates,and is a key downstream enzyme of the plant benzyl acetate bio-synthesis pathway.CpBEAT3 is a key gene regulating the biosynthesis of benzyl acetate in leaves of Chimonan-thus praecox.In order to elucidate the transcriptional regulatory mechanisms of CpBEAT3 in biosynthesis of benzyl acetate in C.praecox,the promoter region sequence of CpBEAT3 was cloned in this study.Its structural features such as core promoter region,cis-acting elements and CpG islands were predicted and analyzed using bioinformatics softwares and the upstream regulatory factors which might bind to CpBEAT3 gene were screened through yeast one-hybrid technology.The results indicated that the 2 115 bp of CpBEAT3 gene core promoter region might be located at-1 844～1 794 bp,which had a CpG island with the length of 410 bp located be-tween-1 869 and-1 459 bp,and contained various cis-acting elements related to light response,hormone regulation and stress,as well as multiple transcription factor binding sites.One MYB class of transcription fac-tor was obtained through yeast one-hybrid technology,which could bind to the promoter of CpBEAT3.
Chimonanthus praecoxCpBEAT3 geneBenzyl acetatePromoterYeast one-hybridInter-acting protein
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