小麦四个抗白粉病基因的KASP标记检测
KASP Marker Detection of Four Powdery Mildew Resistant Genes in Wheat
李玮 1宋国琦 1张迎迎 2李玉莲 1张淑娟 1张荣志 1李吉虎 1高洁 1李根英1
作者信息
- 1. 山东省农业科学院作物研究所/农业部黄淮北部小麦生物学与遗传育种重点实验室/小麦玉米国家工程实验室,山东 济南 250100
- 2. 山东农业工程学院农业科技学院,山东 济南 250100
- 折叠
摘要
为提高Pm13 的检测效率,了解Pm21、PmV、Pm12 和Pm13 共4 个小麦抗白粉病基因在山东小麦育种中的利用情况,本研究通过测序和引物设计成功转化了Pm13 的KASP标记,并采用 4 个基因的KASP标记对2022-2023 年山东省小麦高产区试组和强筋区试组的 140 份参试品系和 2 份区试对照品种进行检测,结果显示 140 份材料均不含上述 4 个基因,表明它们在山东小麦育种中应用较少.本研究可为利用KASP标记开展小麦抗白粉病基因的分子标记辅助选择提供参考,促进其育种应用.
Abstract
In order to improve detection efficiency of Pm13,and to know the utilization status of four mildew resistant genes as Pm21,PmV,Pm12 and Pm13 in wheat breeding programs of Shandong Province,KASP marker of Pm13 was successfully transformed by PCR sequencing and primer designing.The KASP markers of the four genes were employed to detect 140 lines and 2 control cultivars of wheat in the 2022-2023 regional trails of Shandong,belonging to high yield group and high quality group.The result showed that none of the four genes were detected in any of the 140 materials,which indicated that these genes were rarely used in wheat breeding of Shandong Province.This study could provide references for KASP marker-assisted selec-tion of wheat powdery mildew resistant genes and promote their utilization in wheat breeding.
关键词
小麦/抗白粉病基因/Pm13/KASP标记/分子标记辅助选择Key words
Wheat/Powdery mildew resistant gene/Pm13/KASP marker/Marker-assisted selection引用本文复制引用
基金项目
山东省农科院农业科技创新工程项目(CXGC2023A01)
山东省重点研发计划(2019LZGC009)
山东省重点研发计划(2022LZGCQY002)
出版年
2024
国家科技期刊平台
NSTL
万方数据