麦穗干插花加工型小麦种质资源的筛选
Screening of Wheat Resources Suitable for Processing Wheatear Flower
周国雁 1何思柳 2伍少云 1陈丹 1武晓阳 1刘圆圆 1白晓东 3蔡青1
作者信息
- 1. 云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业农村部西南作物基因资源与种质创制重点实验室,云南 昆明 650205
- 2. 普洱学院,云南 普洱 665000
- 3. 武定县猫街镇农业农村服务中心,云南 武定 651607
- 折叠
摘要
本研究以麦穗干插花(以下简称麦穗花)加工企业认可的云生 1 号、云麦 53 号品种为对照,采用芒长、穗长和茎秆强度 3 个加工企业认为影响麦穗花加工和销售的重要指标,对 42 份长芒小麦种质资源进行性状筛选与评价,并利用文献报道的与小麦茎秆强度相关的SSR分子标记对参试资源进行茎秆强度辅助选择,挑选出在农艺性状和分子遗传学上都适合加工麦穗花的优异种质资源,为云南干花产业发展提供新的原材料.结果表明,通过两年的田间种植观测与数据分析,未发现 3 个性状都较两个对照优异的资源.其中,C6(小黑麦)两年的芒长都较两对照显著优异且穗长和茎秆强度差异不明显或也显著优异,是比两个对照更适合加工麦穗花的优异资源;其次是C3 和C9,两年的穗长和茎秆强度都显著优于或与两个对照差异不显著,也是加工麦穗花的较理想资源;C4、C5、C7 和C8 的穗长较云麦 53 号显著优异,芒长和茎秆强度则差异不显著或也显著优异.对茎秆强度的SSR分子标记辅助选择结果表明,C4、C6 可能携带有与云麦 53 号相同的BARC59、BARC134 和WMC48 位点以及与云生 1 号相同的BARC59 位点,C3、C5、C8 也可能携带与云麦 53 号相同的BARC59、BARC134 和WMC48 位点以及与云生 1 号相同的BARC59、BARC358 位点,C9 可能携带有与云麦 53 号相同的BARC59、BARC134 位点以及与云生 1 号相同的BARC59、WMC48 位点.但是,用这些引物获得的分子标记结果与表型评价结果之间的一致性都在 50%及以下,可能这些分子标记都不适宜用作供试小麦材料茎秆强度的辅助筛选.今后还需进一步筛选适合评价麦穗花加工型小麦种质资源的指标及分子标记,为选育满足生产需求的优良小麦品种提供技术支持.
Abstract
In order to screen suitable wheat resources for processing dry wheatear flower arrangement(wheatear flower)both in agronomic traits and molecular genetics and provide new raw materials for serving the development of dry flower industry in Yunnan,the current study took two wheat varieties Yunsheng 1 and Yunmai 53 as controls and three traits including awn length,spike length and stem strength which were ap-proved by the wheatear flower processing enterprises as important indicators.The traits in 42 long-awned wheat germplasm resources were measured and evaluated,and the trait of stem strength was selected using related SSR molecular markers reported in literatures.The results showed that after two years of observation in field and data analysis,no material was found with all the three traits superior to the two control varieties.C6(triti-cale)was superior to the two controls in awn length,and the difference was not obvious or excellent in spike length and stem strength,which was one excellent material more suitable for processing wheatear flower.Be-sides,C3 and C9 were also more suitable resources for processing wheatear flower because the values of spike length and stem strength were better than or not obviously different from those of the two controls.C4,C5,C7 and C8 were also suitable for processing wheatear flower because the spike length was significantly better than that of Yunmai 53,and the awn length and stem strength had no obvious or ecellent differences.The results of SSR molecular marker-assisted selection for stem strength showed that C4 and C6 might have the same SSR lo-ci of BARC59,BARC134 and WMC48 as Yunmai 53 and the same locus of BARC59 as Yunsheng 1.C3,C5,C8 might have the same loci of BARC59,BARC134 and WMC48 as Yunmai 53 and the same loci of BARC59 and BARC358 as Yunsheng 1.C9 might have the same loci of BARC59 and BARC134 as Yunmai 53 and the same loci of BARC59 and WMC48 as Yunsheng 1.However,these primers might not be suitable as molecular markers for stem strength selection,because the consistency between the results of markers and phenotypic e-valuation was less than 50%.In the future,it would be necessary to screen indicators and molecular markers for evaluating wheat germplasm resources for processing wheatear flower,so as to provide technical supports for breeding wheat varieties to meet the production requirements.
关键词
麦穗干插花/小麦种质资源/农艺性状/分子标记辅助选择Key words
Dry wheatear flower arrangement/Wheat germplasm resources/Agronomic traits/Molecular marker-assisted selection引用本文复制引用
基金项目
国家科技资源共享服务平台——国家作物种质资源库项目(NCGRC2022-030)
云南省科技重大专项(生物医药)(202002AA100007)
云南省全国农作物种质资源普查与收集行动财政专项(第三次)(2022)(YAASZYPC01)
出版年
2024
国家科技期刊平台
NSTL
万方数据