Comprehensive Evaluation on Saline-Alkali Tolerance of 35 Mung Bean Germplasm Resources during Germination Period and Analysis of Saline-Alkali Tolerant Mechanism
In order to clarify the evaluation indexes of saline-alkali tolerance for mung bean germplasm resources during germination period,35 mung bean resources were used as test materials and treated with 40 mmol·L-1 saline-alkali mixture(NaCl∶Na2 CO3∶Na2 SO4∶NaHCO3=1∶1∶9∶9,pH value8.9)during germination period.Fourteen indexes related to germination such as germination potential,germination rate and radicle length were determined.The principal component analysis,membership function analysis,cluster analysis and correlation analysis were conducted,the activities of antioxidant related enzymes were measured,and the sa-line-alkali tolerant germplasm resources of mung bean were screened.The results showed that there were differ-ences in the germination indexes among different mung bean germplasm materials under saline-alkali stress.The cumulative contribution rate of five principal components was up to 83.330%based on the principal com-ponent analysis.Results of membership function analysis showed that LSS-13 and Xinyu 1 were germplasms with relative higher saline-alkali tolerance.Cluster analysis showed that the 35 mung bean resources could be divided into 5 categories,among which,Xinyu 1,Hei Mung bean(2020),Taolü 8,Chilü 6 and Taolü 9 were clustered in categoryⅠ,which were strong saline-alkali tolerant germplasms.Based on the principal com-ponent and correlation analyses,the total fresh weight,cotyledon fresh weight,radicle fresh weight,radicle dry weight and radicle length could be used as important indexes for salt-alkali tolerance evaluation of mung bean during germination.Compared to the control,the activities of peroxidase,superoxide dismutase,catalase and ascorbate peroxidase increased significantly in cotyledon and radicle of mung bean under salt-alkali stress.The results of RAPD analysis showed that saline-alkali stress could cause genomic DNA damage in mung bean.This study could provide theoretical bases for breeding mung bean varieties for saline-alkali areas and analysis of physiology and molecular mechanisms of saline-alkali tolerance.
Mung beanGermination periodSaline-alkali stressComprehensive evaluationPhysiolog-ical responseNucleic acid damage repair
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