Analysis on Bioinformatics Characteristics and Expression Pattern of LeGlyⅠ Gene in Tomato
The plant may produce excessive toxic aldehydes such as methylglyoxal(MG)when facing adversity stress,and glyoxal enzyme system can effectively clear the excessive MG.Glyoxalase I(GlyⅠ)as the first enzyme in the cascade reaction plays an important role.In this study,LeGlyⅠgene was successfully cloned from the seedlings of tomato'Heinz 1706',and its bioinformatics characteristics,expression pattern and subcellular localization were analyzed.The results showed that the total length of LeGlyⅠCDS sequence was 489 bp,which encoding 162 amino acids.The LeGlyⅠ was predicted to be a hydrophilic stable protein with molecular formula as C798 H1226 N214 O235 S4,molecular weight as 17 706.05 Da,isoelectric point as 6.51,GRAVY coefficient as-0.386 and instability index as 39.32,and it had a VOC-like domain.The promoter se-quence of LeGlyⅠcontained a variety of cis-acting elements,including cis-acting elements necessary for anae-robic induction,light response elements,low-temperature response elements,drought response elements,and hormone response elements such as methyl jasmonate,abscisic acid,auxin and gibberellin.Through qRT-PCR analysis,it was found that LeGlyⅠexpressed in roots,stems and leaves of tomato with the highest expression level in leaves,and the expression could be affected by oxidative stress.The subcellular localization results showed that LeGlyⅠprotein was mainly localized in mitochondria and nucleus.These results could provide the-oretical bases for further research on the function and molecular mechanism of LeGlyⅠgene in tomato resistant to stresses.
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