Identification of Alfin-Like Family Genes and Their Expression Analysis under Salt Stress in Malus doumeri'Royalty'
The Alfin-like(AL)transcription factor family play important regulatory roles in response to abiotic stresses.In this study,the homologous alignment method was used to search and identify the AL tran-scription factor family genes of Malus doumeri'Royalty',the bioinformatics characteristics were systematically analyzed,and their expression patterns under salt stress were analyzed by qRT-PCR method in order to provide a theoretical basis for revealing the physiological function of AL family in response to salt stress.The results showed that 11 MdAL genes were identified from the genome of M.doumeri'Royalty',which were distributed on 6 chromosomes and named from MdAL1 to MdAL11,respectively.The MdAL proteins had highly conserved Alfin domain and PHD zinc finger domain,containing 4～10 introns.The upstream promoter region contained a large number of cis-acting elements relating to plant hormone and abiotic stress responses,and gene fragment replication events played a crucial role in the expansion of the MdAL gene family.Transcriptome analysis showed that MdALs were mainly expressed highly in late growth period of M.doumeri'Royalty'.Furthermore,the expression patterns of the MdAL genes were different under salt stress.The expression level of MdAL3,MdAL4 and MdAL6 genes were up-regulated under salt stress,especially MdAL4,whose expression level in-creased significantly with the prolongation of salt stress time.In summary,the AL transcription factor family of M.doumeri'Royalty'was closely related to plant hormone and abiotic stresses,especially the MdAL4 gene,which strongly responded to salt stress.All these results could lay the foundation for improving M.doumeri'Royalty'germplasms using genetic engineering technologies.
Malus doumeri'Royalty'Alfin-like family geneBioinformatics analysisFunctional iden-tificationSalt stressGene expression
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