Provenance Diversity Analysis and Evaluation of Polygonatum cyrtonema Hua Based on Quality Traits and Mineral Element Content
In order to study the genetic diversity of quality traits and mineral elements of Polygonatum cyrtonema Hua germplasm resources and screen high-quality germplasms,43 P.cyrtonema germplasm re-sources in China were collected,and the contents of 7 quality traits and 15 mineral elements were determined,and a comprehensive evaluation was conducted by diversity analysis,correlation analysis,principal component analysis,clustering analysis and membership function method.The results showed that the contents of polysac-charide,total ash,alcohol-soluble extract,water,and some heavy metals and harmful elements in the germ-plasm resources met the standards of China Pharmacopoeia(2020 edition).The variation coefficient of quality traits was 10.4%to 46.7%,and the genetic diversity index was 1.600 to 2.015.The variation coefficient of mineral elements was 23.6%to 92.7%,and the genetic diversity index was 1.526 to 2.006.Correlation analy-sis results showed that significant(P<0.05)or extremely significant(P<0.01)levels were reached in a total of 76 pairs of indicators.The correlation between mineral elements was complex but the overall synergistic effect was strong,and the inhibition effect of mineral elements on quality traits was stronger.Eleven mineral elements were simplified into three principal components by principal component analysis with the comulative contribution rate as 66.71%,and K,Zn,Fe and P were screened out as the characteristic elements for evalu-ating the germplasm resources of P.cyrtonema.The 43 germplasm resources were divided into four groups at the distance of 100 when analyzed by systematic clustering,and the result was basically consistent with that of comprehensive evaluation by membership function method,and the top ten high-quality provenances were S33,S27,S29,S28,S31,S10,S17,S36,S32,and S20,respectively.In conclusion,there were obviously variation in quality traits and mineral elements among the germplasm resources of P.cyrtonema,which showed rich genetic diversity.The results could provide a reference for breeding high quality P.cyrtonema.
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