首页|枯草芽孢杆菌对土壤真菌群落结构及草莓根腐病的调控作用

枯草芽孢杆菌对土壤真菌群落结构及草莓根腐病的调控作用

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
为探究施用枯草芽孢杆菌对土壤真菌群落结构和作物生长的调控作用，本研究采用高通量测序技术分析枯草芽孢杆菌对草莓根际土壤真菌群落多样性和群落结构的影响，通过田间小区试验测定其对草莓根腐病的防治效果和对草莓的促生长作用。结果表明，施用枯草芽孢杆菌显著提高草莓根际土壤真菌多样性，Shannon指数、Ace指数和Chao指数显著上升，Simpson指数显著下降;门水平上子囊菌门(Ascomycota)相对丰度明显增加，担子菌门(Basidiomycota)和被孢菌门(Mortierellomycota)相对丰度明显减少;属水平上优势菌属毛壳菌属(Chaetomium)相对丰度明显增加，被孢霉属(Mortierella)、Tausonia和织球壳菌属(Plectosphaerel-la)相对丰度明显降低;枯草芽孢杆菌能有效降低草莓根腐病的发病率，定植 2 个月内的防治效果在 70%以上，并显著提高草莓冠径、叶面积及叶绿素含量等生长指标值。综上，枯草芽孢杆菌能够增加草莓根际土壤中有益菌相对丰度，减少致病菌相对丰度，优化土壤真菌群落结构，有效防治草莓根腐病，促进草莓健康生长。
Regulation of Bacillus subtilis on Soil Fungal Community Structure and Root Rot of Strawberry
In order to explore the regulation of Bacillus subtilis on soil fungal community structure and crop growth,high-throughput sequencing technology was used to analyze the effects of B.subtilis on fungal di-versity and community structure in strawberry rhizosphere soil,and the effects on controlling strawberry root rot and promoting strawberry growth were determined by field plot experiment.The results showed that the applica-tion of B.subtilis significantly increased the fungal diversity in strawberry rhizosphere soil.The Shannon index,Ace index and Chao index increased significantly,and the Simpson index decreased significantly.At phylum level,the relative abundance of Ascomycota increased significantly,while that of Basidiomycota and Mortierel-lomycota decreased significantly.At genus level,the relative abundance of Chaetomium increased significant-ly,while that of Mortierella,Tausonia and Plectosphaerella decreased significantly.B.subtilis could effectively reduce the incidence of strawberry root rot with the control effect higher than 70%within 2 months of planting,and significantly increased the growth indexes such as canopy diameter,leaf area and chlorophyll content.Comprehensive analysis showed that B.subtilis could increase the relative abundance of beneficial fungi but re-duce that of pathogenic fungi in strawberry rhizosphere soil,so optimized the soil fungal community structure,effectively controlled strawberry root rot and promoted the healthy growth of strawberry.

Bacillus subtilisStrawberryHigh-throughput sequencingFungi community structureStrawberry root rot

李宝庆、石亚冰、凌凡舒、徐丹、孙卿、刘玉珊、韩庆典

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临沂市农业技术推广中心,山东 临沂 276000

临沂大学农林科学学院,山东 临沂 276000

浙江大学山东(临沂)现代农业研究院,山东 临沂 276000

枯草芽孢杆菌 草莓 高通量测序 真菌群落结构 草莓根腐病

山东省生态农业产业技术体系项目山东省农业重大应用技术创新项目浙江大学山东(临沂)现代农业研究院服务地方经济发展项目临沂大学社会服务项目

SDAIT-30-122015NY212ZDNY-2021-FWLY02004HX230195

2024

10.14083/j.issn.1001-4942.2024.06.011

山东农业科学
山东省农业科学院,山东农学会,山东农业大学

山东农业科学

CSTPCD北大核心
影响因子：0.578
ISSN：1001-4942
年,卷(期)：2024.56(6)