Bioinformatics and Expression Analyses of Juvenile Hormone Epoxide Hydrolase Genes JHEH1 and JHEH1-2 in Aethina tumida
Juvenile hormone epoxide hydrolase(JHEH)is one of the key enzymes regulating juvenile hormone(JH)metabolism,making it a potential target for selective insecticide.The physicochemical proper-ties,structural features and phyletic evolution of the encoding proteins of JHEH1 and JHEH1-2 in Aethina tu-mida were analyzed using bioinformatics methods.The relative expression levels of JHEH1 and JHEH1-2 in o-varies at various developmental stages of A.tumida adults(not emerged,1-day-old,2-day-old,and 3-day-old)and tissues of not-emerged adults(ovary,fat body,leg,thorax and head)were detected using real-time quantitative PCR.JHEH1 was predicted to encode a protein consisting of 460 amino acids with the isoelectric point(pI)as 8.06 and the molecular formula as C2425 H3707 N603 O660 S16.JHEH1-2 was predicted to encode 461 amino acids with the pI as 8.70 and the molecular formula as C2420 H3713 N597 O656 S15.Both had the molecular weight of 52 kDa and possessed the N-terminal structure of an epoxide hydrolase.The phylogenetic tree indica-ted that the JHEH1 gene of A.tumida clustered with JHEH gene from Anpoploppor aglabripennis.In contrast,JHEH1-2 of A.tumida was more conserved,and the confidence level of its branch was relatively low.Real-time quantitative PCR analysis revealed that both JHEH1 and JHEH1-2 expressed in various tissues and at dif-ferent developmental stages of A.tumida adults,and their expression levels in ovaries of the not-emerged a-dults were significantly higher(P<0.05),which suggested a crucial role of JHEH in the ovarian development process of this species.These findings could provide the theoretical basis for further exploring the regulatory mechanisms of JHEH in growth and development of A.tumida.
国家科技期刊平台
NSTL
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