松树赤枯病玫瑰拟盘多毛孢菌的鉴定及生物学特性研究
Identification and Biological Characteristics of Pestalotiopsis rosea Causing Pine Needle Blight
韩凤英 1亓玉昆 2杨慧 1秦永梅 1周丽颜 1李星仪 1李海燕 3刘敏 1王清海2
作者信息
- 1. 山东农业工程学院,山东 济南 250100
- 2. 山东省林业科学研究院,山东 济南 250014
- 3. 济南市公园发展服务中心,山东 济南 250001
- 折叠
摘要
本研究对从山东威海双岛林场采集的松树枯萎样本进行分离培养、单孢纯化、致病性测定,得到一株能引起松树赤枯病的病原菌,编号为RCBHL.经形态学观察和多基因(ITS、TEF-1、TUB2)系统发育分析,将菌株鉴定为玫瑰拟盘多毛孢菌(Pestalotiopsis rosea).生物学特性测定结果表明,该菌株最适生长温度为20～25℃,最优生长培养基为PDA培养基,最适生长pH值为 6.0,最适光照条件为 24h光照.该研究结果可为松树赤枯病的防治提供理论依据.
Abstract
In this experiment,the samples of pine needle blight were collected from the Shuangdao For-est Farm in Weihai,Shandong Province,and a pathogenetic strain RCBHL was obtained after separation and culture,single spore purification and pathogenicity identification test.According to morphological characteris-tics and multilocus phylogenetic(ITS,TEF-1 and TUB2)analyses,it was identified as Pestalotiopsis rosea.The testing results of biological characteristics showed that the optimum cultural conditions for P.rosea RCHBL included 20～25℃,6.0 of pH value,PDA culture medium and 24 h light.The research results would provide the theoretical basis for prevention and control of pine needle blight.
关键词
松树赤枯病/分离纯化/病原鉴定/生物学特性Key words
Pine needle blight/Separation and purification/Pathogen identification/Biological charac-teristics引用本文复制引用
基金项目
中央林业改革发展资金防灾减灾项目(鲁财资环指[2021]27号)
2022年山东省本科教学改革研究项目(Z2022211)
山东省"黄河流域生态保护和教育高质量发展"教育教学专项课题(2022HHZX005)
出版年
2024
国家科技期刊平台
NSTL
万方数据