Identification and Biological Characteristics of Pestalotiopsis rosea Causing Pine Needle Blight
In this experiment,the samples of pine needle blight were collected from the Shuangdao For-est Farm in Weihai,Shandong Province,and a pathogenetic strain RCBHL was obtained after separation and culture,single spore purification and pathogenicity identification test.According to morphological characteris-tics and multilocus phylogenetic(ITS,TEF-1 and TUB2)analyses,it was identified as Pestalotiopsis rosea.The testing results of biological characteristics showed that the optimum cultural conditions for P.rosea RCHBL included 20～25℃,6.0 of pH value,PDA culture medium and 24 h light.The research results would provide the theoretical basis for prevention and control of pine needle blight.
Pine needle blightSeparation and purificationPathogen identificationBiological charac-teristics
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