Development of Fluorescence in situ Hybridization(FISH)Markers Specific for Chromosome 1HtS of Elymus trachycaulus
Wheat-Elymus trachycaulus 1HtS.1BL Robertson translocation is highly resistant to wheat stripe rust and leaf rust,and is an excellent gene source for wheat genetic improvement.We previously in-duced this translocation line using a Chinese Spring(CS)ph1b gene mutant and obtained a batch of induced offspring.In order to accurately identify the wheat-E.trachycaulus small chromosome fragment translocation lines that 1HtS involved,it is necessary to establish a fluorescence in situ hybridization(FISH)marker that can cover E.trachycaulus 1Ht S.In this study,21 wheat and its wild species probes,61 new wheat 1BS chro-mosome probes developed based on the CS genome sequence,and 40 probes developed based on a simple triple base repeat sequence were used to perform non-denaturing FISH analysis of wheat-E.trachycaulus 1HtS.1BL Ro-bertson translocation.The results showed that 36 probes including B74,B76 and B77(33 of which were newly developed),had hybridization signals on the 1HtS chromosome,and the hybridization signals could be divided into five types:only at the terminal region of 1Ht S,only at the centromere,at the centromere and near the centromere simultaneously,covering about 3/4 of 1Ht S,and covering most of regions of 1HtS.Therefore,some probes used alone or in combination with multiple probes could cover the whole 1HtS chromosome,which could meet the identification purpose of relative small chromosome fragment translocation lines,and pro-vide a new detection method for tracking E.trachycaulus chromatin in wheat background.
Elymus trachycaulus1HtS chromosomeProbe,Fluorescence in situ hybridization(FISH)marker
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