Cloning and Functional Verification of Sorghum Deep Rooting Gene SbDRO1
Drought has seriously affected growth and yield of crops.The structure and distribution of roots were key factors in plant response to drought stress.As a crop with strong drought resistance,exploring drought resistance related genes in sorghum plays an important role in improving crop drought resistance.In this study,the deep rooting gene SbDRO1 was cloned from sorghum,and then its bioinformaticsan alysis was conducted,and its function was validated in Arabidopsis.The results indicated that the total length of CDS of SbDRO1 gene was 759 bp encoding 252 amino acids.Overexpression of SbDRO1 gene could significantly improve Arabidopsis survival rate under drought conditions,and the root system of transgenic Arabidopsis was significantly developed compared to the wild type.Further research found that the expression of genes related to auxin synthesis,polar auxin transport and stress resistance in transgenic Arabidopsis was significantly higher than that in wild type,which might be the main molecular mechanism of SbDRO1 gene improving drought resistance of transgenic Arabi-dopsis.These results could provide potential genetic resources for crop drought resistance improvement.
万方数据
维普
