Cloning and Bioinformatics Analysis of CsWRKY20 Gene and Its Expression Response to High Temperature Stress in Cucumber
Cucumber is one of the major vegetables in facility greenhouse in China.High temperature can cause cucumber growth to be stunted and resistance to be reduced,resulting in yield and quality decline.In this study,a WRKY transcription factor,CsWRKY20,was cloned from the cDNA of cucumber leaves using homologous cloning technology.The CDS length of the CsWRKY20 was 1 602 bp,encoding 533 amino acids.The molecular weight of the CsWRKY20 protein was 132 959.43 Da,and the isoelectric point was 4.97.The CsWRKY20 was predicted to be localized in nucleus,contained two typical conserved WRKY domains,and had the closest sequence homology to CmWRKY20 of muskmelon.The real-time quantitative PCR analysis showed that CsWRKY20 was expressed in both nutrient and reproductive organs,with the highest expression level in cucumber fruit spines.High temperature stress resulted in significant increase of CsWRKY20 expression level at 1 hour,and then decreased and recovered to the initial expression level after 6 hours.The results of this study could provide a theoretical basis for breeding high temperature-tolerant variety of cucumber.
CucumberWRKY transcription factorBioinformatics analysisHigh temperature stressGene expression analysis
万方数据
维普
