Transcriptome Analysis of Panax quinquefolius Infected by Rust Root Rot Disease
To explore the effects of rust root rot disease on gene expression patternand provide basic data for further understanding the mechanism of resistance to this disease in Panax quinquefolius,the differentially expressed genes(DEGs)in both healthy and rusty P.quinquefolius roots and leaves were analyzed by RNA-seq technology,and the functional clustering analysis of the DEGs was carried out.The results were as follows.Compared to the control group,a total of 8 642 DEGs were identified in the BL-vs-AL group,among which,4 463 were up-regulated and 4 179 were down-regulated;a total of 5 308 DEGs were identified in the BR-vs-AR group,of which,2 609 were up-regulated and 2 699 were down-regulated.GO analysis indicated that most DEGs were significantly enriched in cellular processes,metabolic processes,cell,catalytic activities,and oth-er aspects.KEGG analysis showed that DEGs were mainly enriched in phenylpropanoid biosynthesis,unsatu-rated fat acid biosynthesis,MAPK signaling pathway,and other metabolic pathways related to plant disease re-sistance.Besides,116 transcription factors were predicted in the two sets of shared DEGs,which were respec-tively classified into 25 transcription factor families.The 7 randomly selected DEGs were verified by qRT-PCR,and the expression trends were consistent with those of transcriptome sequencing.This study clarified the expression profiles of genes and related metabolic pathways of P.quinquefolius infected by rust root rot disease in heterogeneous habitats,which could provide a certain references for molecular mechanism study on the re-sistance of P.quinquefolius to rust root rot disease.
万方数据
维普
