Composition of Symbiotic Bacteria in Eriosoma lanigerum(Hemiptera:Pemphigidae)and Effects of High Temperature on Their Diversity
Eriosoma lanigerum is an important pest in apple orchards,and high temperature is unfavor-able for its occurrence.To clarify the composition of symbiotic bacteria in E.lanigerum and whether the symbi-otic bacteria mediating the effect of high temperature on the fitness of E.lanigerum,the Illumina MiSeq se-quencing platform was used to determine the 16S rDNA sequences of the symbiotic bacteria in E.lanigerum,and the species composition and the effects of high temperature on diversity and relative abundance of symbiot-ic bacteria were analyzed.Through sequencing,a total of 823 258 high-quality sequences and 5 139 OTUs were obtained.The Proteobacteria was the dominant phylum with the Gammaproteobacteria,Bacteroidia and Alphaproteobacteria being dominant classes,and the Bacteroidales,Betaproteobacteriales and Enterobacteria-les being dominant orders.The symbiotic bacteria of the nymphs were highly similar among instars,showing significant differences from those of the adults.Specifically,the relative abundance of Pectobacterium(29.76%)and Ochrobactrum(13.68%)in adults was higher than that in nymphs,while that of Thermus(2.72%)and Cupriavidus(1.42%)was lower than that in nymphs.Under high temperature,the diversity of symbiotic bacteria within E.lanigerum bodies significantly decreased.Concurrently,the relative abundance of the entomopathogenic Pseudomonas genus gradually increased with temperature rising.In contrast,the relative abundance of the Pectobacterium genus,which was advantageous for host's feeding,and the Acinetobacter ge-nus,which was crucial for survival,gradually decreased with temperature increasing.It was speculated that the changes of the above three species of symbiotic bacteria should be key to the reduction in fitness of E.lan-igerum under high temperature.This study clarified the species composition of symbiotic bacteria in E.lan-igerum and the effect of high temperature on them,which would provide a theoretical basis for the interaction between symbiotic bacteria and hosts,and lay the theoretic foundation for elucidating the mechanism of high temperature reducing the fitness of E.lanigerum.
万方数据
维普
