Effects of Combined Application of Biochar and Nano-Silica on Photosynthetic Fluorescence Characteristics of Oilseed Rape
In order to explore the effects of combined application of biochar and nano-silica on photosyn-thetic fluorescence characteristics of oilseed rape leaves,the pot experiment was conducted by setting four treatments as CK(control),C(biochar),SI(nano-silica)and C+SI(combined application of biochar and nano-silica).The photosynthetic fluorescence characteristics and contents of chlorophyll,soluble sugar and soluble protein in oilseed rape were determined and analyzed.The results showed that compared with CK,the application of biochar and nano-silica could increase the net photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)of oilseed rape leaves,and the effect of their combined application was the best;the overall performance of each index was C+SI>SI>C>CK.Single application of biochar or nano-silica could significantly improve the photochemical quenching coefficient(qP)and PSⅡ actual photochemical quantum efficiency(ΦPSⅡ),and the effect of the former was better on qP,while that of the latter was better on ΦPSⅡ;combined application had higher effect on each fluorescence parameter compared with single appli-cation of biochar or nano-silica,and significantly improved the electron transport efficiency(ETR),variable fluorescence(Fv),maximum fluorescence(Fm)compared with CK.The combined application of biochar and nano-silica could significantly increase the chlorophyll,soluble sugar and soluble protein contents in oilseed rape leaves;single application of biochar significantly increased the content of chlorophyll a and soluble pro-tein,and single application of nano-silica only significantly increased the content of soluble protein.In summa-ry,the combined application of biochar and nano-silica had the best effect,which could significantly improve the photosynthetic efficiency of leaves,optimize the energy transport and utilization of the reaction center,and promote the growth and development of oilseed rape plants.
万方数据
维普
