3种鸡源乳酸菌抑制大肠杆菌生物被膜形成的比较研究

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中文摘要：试验旨在研究鼠李糖乳杆菌、戊糖片球菌和口乳杆菌 3 种鸡源乳酸菌对大肠杆菌生物被膜生成的影响。利用置片法对供试菌株培养 48 h，定性观察细菌生长与生物被膜的形成;利用 96 孔微板法定量检测 4种细菌生长的OD590值，评价抑制生物被膜生成的效果。结果表明，大肠杆菌培养 12h开始形成生物被膜，24 h形成典型的生物被膜，并伴有交织链接的生物被膜骨架;3 种乳酸菌培养36h均未见生物被膜骨架，OD590值显示鼠李糖乳杆菌生物被膜厚度优于戊糖片球菌、口乳杆菌。与大肠杆菌共培养发现，3 种乳酸菌全菌液和上清液均能显著抑制大肠杆菌生物被膜的生成，抑制效果鼠李糖乳杆菌>口乳杆菌>戊糖片球菌。结果可为探索乳酸菌拮抗大肠杆菌感染机制提供参考。

外文标题：Comparison of Three Lactic Acid Bacteria from Chickens on Inhibiting Escherichia coli Biofilm Formation

外文摘要：This study aimed to explore the effects of Lactobacillus rhamnosus,Pediococcus pentosaceus and Lactobacillus oris on biofilm formation of Escherichia coli.After cultured for 48 hours by using the film placement method,the bacterial growth and biofilm formation were qualitatively observed,and the OD590 value of the four bacterial strains were quantitatively detected by 96-well microplate method to evaluate their effects on inhibiting biofilm formation.The results showed that E.coli began to form biofilm at 12 h of culture,and the typical biofilm was formed at 24 h accompanied by interwoven and linked biofilm skeleton.No biofilm skel-eton was found after 36 h of co-culture with the three species of lactic acid bacteria.The OD590 value showed that the biofilm thickness of L.rhamnosus was better than that of P.pentosaceus and L.oris.The co-culture re-sults showed that the whole bacterial solution and supernatant of the three species of lactic acid bacteria could significantly inhibit the formation of E.coli biofilm,and their inhibitory effects showed L.rhamnosus>L.oris>P.pentosaceus.These results could provide references for exploring the mechanism of lactic acid bacteria an-tagonizing E.coli infection.

外文关键词：

Lactobacillus rhamnosusPediococcus pentosaceusLactobacillus orisEscherichia coliBio-film formation

作者：

赵桂省、庞天津、陈甫、刘玮、李福伟、邱慧玲

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作者单位：

济南市畜牧技术推广站,山东济南 250306

青岛农业大学海都学院,山东莱阳 265200

青岛农业大学动物医学院,山东青岛 266109

山东省农业科学院家禽研究所/济南市家禽种质创新与健康养殖重点实验室,山东济南 250100

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关键词：

鼠李糖乳杆菌戊糖片球菌口乳杆菌大肠杆菌生物被膜形成

基金：

山东省家禽产业技术体系项目国家现代农业产业技术体系项目山东省良种工程项目中央引导地方科技发展专项科技创新2030-重大项目泰山产业领军人才工程专项山东省科技型中小企业创新能力提升工程项目

项目编号：

SDAIT-11-07CARS-402022LZGC013YDZX20230342023ZD04052TSCX2023120572022TSGC2230

出版年：

2024

DOI：

10.14083/j.issn.1001-4942.2024.07.019

山东农业科学

山东省农业科学院,山东农学会,山东农业大学

山东农业科学

CSTPCD北大核心

影响因子：0.578

ISSN：1001-4942

年,卷(期)：2024.56(7)