Identification and Expression of Vitellogenin in Oriusnagaii
In this study,we analyzed the structure,prokaryotic expression,antibody preparation and spatio-temporal dynamic expression of vitellogenin(Vg)from Orius nagaii,aiming to provide theoretical bases for study of Vg in insects.The full length of OnVg gene was cloned by RACE method,and the OnVg protein was analyzed by biological information website which had three conserved domains including lipoprotein N-ter-minal domain,unknown function domain and von Willebrand factor domain.The prokaryotic expression vector of OnVg,pET-32a-c(+)-OnVg,was constructed and transferred into Escherichia coli to induce expression.Antibody of anti-OnVg was prepared by immunizing Kunming mice.Western blot results showed that anti-On-Vg antibody could bind specifically to OnVg protein.The spatio-temporal dynamic expression of Vg gene in sta-ges of nymphs,adults,and different adult tissues were detected by qPCR,and the results showed that the ex-pression level of Vg gene was the lowest in nymphs,but increased in adults,and reached the highest level on the 3rd day of adult.In adults,it expressed the highest in abdomen,followed by intestine and ovary.The re-sults of this study could provide theoretical supports for exploring the mechanism of reproductive regulation of O.nagaii.
万方数据
维普
