利用CRISPR/Cas9基因编辑片段敲除技术创制玉米多样化等位变异
Engineering Diversified Allelic Variation in Maize by Fragment Knockout Using CRISPR/Cas9 Genome Editing
张茂林 1王娟 1严佳丽 2何春梅 1徐倩 1刘铁山 1董瑞 1刘春晓 1关海英 1刘强 1汪黎明 1曾廷儒1
作者信息
- 1. 山东省农业科学院玉米研究所/小麦玉米国家工程实验室/农业部黄淮海北部玉米生物学与遗传育种重点实验室,山东济南 250100
- 2. 华中农业大学作物遗传改良全国重点实验室,湖北武汉 430070
- 折叠
摘要
利用CRISPR/Cas9基因编辑技术创制等位变异已经成为众多植物中增加种质资源多样性的重要手段.提高玉米等谷物中类胡萝卜素含量是发展中国家解决维生素A缺乏症的一种经济方法.本研究以玉米中调控类胡萝卜素合成的基因crtRB1为研究对象,利用双靶标片段缺失性基因编辑技术,在原生质体中对crtRB1的启动子顺式元件进行片段敲除,获得了具有片段缺失和InDel(插入或缺失)的多样化等位变异,这为在玉米中实现稳定的crtRB1启动子元件的缺失突变奠定了基础,对启动子元件的功能研究和玉米的品质改良具有重要意义.
Abstract
Engineering allelic variation by CRISPR/Cas9 gene editing technology has become an impor-tant mean to increase germplasm resources diversity in many plants.Increasing the content of carotenoids in ce-reals such as maize is an economic approach to solve vitamin A deficiency in developing countries.In this stud-y,the crtRB1 gene regulating carotenoid synthesis in maize was chosen as subject,the CRISPR/Cas9 dual-target system was used to perform fragment deletion on the promoters of crtRB1 in maize protoplasts,and then new diversified allelic variations with fragment deletion and InDel(insertion or deletion)were engineered.The results laid a foundation for the engineering of mutations in crtRB1 promoters and was of great significance to the functional research of promoter elements and improvement of maize quality.
关键词
玉米/crtRB1/基因编辑/片段敲除/启动子Key words
Maize/crtRB1/Gene editing/Fragment knockout/Promoter引用本文复制引用
基金项目
山东省自然科学基金面上项目(ZR2021MC084)
农业生物育种重大项目(2023ZD0403002)
出版年
2024
国家科技期刊平台
NSTL
万方数据