Optimization of Cymarin Extraction and Detection Conditions and Analysis of Its Cumulative Effects over Multiple Years
To optimize the extraction and detection conditions of cymarin in Apocynum venetum L.(AV)and clarify the influence of planting years on its cumulative effect,ultrasonic extraction was used to extract cy-marin,and its content was determined by the 3,5-dinitrobenzoic acid-sodium hydroxide colorimetric method.Its antioxidant capacity was detected by ferric reducing/antioxidant power(FRAP)and 1,1-diphenyl-2-trini-trophenylhydrazine(DPPH)methods.The results showed that the absorbance of cymarin standard solution at 540 nm had a good linear relationship with its concentration in the range of 0～1.0 mg/mL(R2=0.992)with sample recovery rate as 99.218％and relative standard deviation(RSD)as 1.909％.Within the investigating range,the analysis results of ethanol concentration,ultrasonic time and liquid-to-solid ratio showed that the highest cymarin content of 639.34 μg/g could be obtained when using 95％ethanol as extraction reagent,the ratio of sample to extraction reagent as 1∶10 g/mL,and ultrasonic extraction for 30 minutes.The distribution of cymarin content in different parts of AV was significantly different among different planting years(3,7 and 10 years),which were sequenced in the order of 7-year leaves＞3-year leaves＞10-year leaves＞3-year stems＞7-year roots＞10-year roots＞3-year stems＞7-year roots＞10-year stems.Comparing between planting years,the AV with 7 planting years had higher cymarin content as 626.25 μg/g;the antioxidant capacity showed 7 years＞3 years＞10 years,and stronger DPPH free radical scavenging ability was found among the three kinds of planting years.Correlation analysis showed that the cymarin content of 7-year AV was positively correlated with the antioxidant capacity.These results could provide bases for cymarin extraction and related functional product development.
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