Effects of High-Carbon Basal Fertilizer Combined with Nitrogen Reduction on Total Carbon,Total Nitrogen and Fungal Community Structure in Tobacco-Planting Soil
In order to study the effect of high-carbon basal fertilizer application on tobacco-growing soil,the field experiment was adopted,and four treatments were set including conventional fertilization of pure ni-trogen 111 kg·hm-2(GCK),high-carbon basal fertilizer 450 kg·hm-2 combined with nitrogen reduction 10%(G3),high-carbon basal fertilizer 750 kg·hm-2 combined with nitrogen reduction 20%(G5)and high-carbon basal fertilizer 1 050 kg·hm-2 combined with nitrogen reduction 30%(G7).The contents of soil total nitrogen(TN)and total carbon(TC)were determined,and soil fungal community structure and their relationships with TN and TC were analyzed by ITS sequencing technology.The results were as follows.(1)G7 treatment in-creased soil TC content and C/N ratio and decreased soil TN content at 60 and 90 days after transplanting.(2)G3 treatment significantly increased soil fungal diversity at 30 and 90 days after transplanting.(3)The application of high-carbon basal fertilizer combined with nitrogen reduction affected the community structure of fungi.In the phylum level,it increased the relative abundance of Zygomycota at 60 days after transplanting and decreased that of Ascomycota.In the genus level,it increased the relative abundance of Trichoderma and decreased that of Fusarium at 60 days after transplanting.(4)The application of high-carbon basal fertilizer combined with nitrogen reduction changed the fungal community structure through influencing soil TC,TN contents and C/N ratio.In conclusion,the application of 1 050 kg of high-carbon basal fertilizer+77.7 kg of pure nitrogen per hectare(G7)had positive effects on soil fungal community structure and soil TC,TN and C/N ratio.
万方数据
维普
