Effect of Biochar on Bacterial Community Structure in Rhizosphere Soil of Tobacco Varieties with Different Resistance to Black Shank Disease
This study aimed to explore the potential effects of adding biochar into the rhizosphere soil of different varieties of tobacco on bacterial community structure,and provide theoretical supports for improving and optimizing regulatory measures for tobacco microenvironment and strategies for comprehensive prevention and control of tobacco black shank disease.A pot experiment was designed with three tobacco varieties,Jingye Huang,NC82 and K326,as research objects.The soil without tobacco cultivation was as a blank control(CK),and eight treatments with and without biochar application were set.High throughput sequencing tech-nology was used to investigate the effects of biochar application on bacterial community structure of rhizosphere soil and the incidence of tobacco black shank disease.The results were as follows.(1)The incidence of black shank disease of Jingye Huang tobacco was significantly higher than that of K326 and NC82 cultivars when without biochar application.After the application of biochar,the incidence of black shank disease of all the va-rieties reduced significantly,and the control effect of Jingye Huang,NC82 and K326 were 56.52%,27.00%and 31.00%,respectively.(2)The Alpha diversity analysis of soil bacterial communities showed that there was significant interaction between biochar and tobacco variety.When without biochar application,the rhizo-sphere soil bacterial diversity of NC82 and Jingye Huang were significantly higher than that of K326.After the application of biochar,the soil bacterial diversity of K326 increased significantly,while NC82 and Jingye Huang showed different degrees of decline.(3)The LEfSe results showed that there were significant differ-ences in dominant flora in rhizosphere soil among different varieties,and the application of biochar made the differences more obvious.(4)The bacterial community structure and composition analyses showed that the bacterial community structure in rhizosphere soil of the three tobacco varieties had significant differences,and no significant changes were observed after the application of biochar except for CK.In summary,the applica-tion of biochar could effectively curb the spread of tobacco black shank disease.The response of rhizosphere soil bacterial structure to the application of biochar varied with different tobacco varieties.Therefore,the influ-ence of variety must be fully taken into account in the process of using biochar to improve soil quality and to-bacco micro-ecological environment.
BiocharBlack shank diseaseTobaccoRhizosphere soilBacterial community structure
万方数据
维普
